基于共振拉曼探针(BCA/Cu^+)的Cu^(2+)定量分析  

Quantitative Determination of Cu^(2+) Based on Resonance Raman Probe(BCA/Cu^+)

作　　者：赵冰[1] 韩晓霞[1] 陈雷[2] 

机构地区：[1]吉林大学超分子结构与材料国家重点实验室,吉林长春130012 [2]吉林师范大学化学学院,吉林四平136000

出　　处：《吉林师范大学学报（自然科学版）》2017年第1期1-5,共5页Journal of Jilin Normal University:Natural Science Edition

基　　金：国家自然科学基金项目(21273091;21403082);吉林省科技发展计划项目(20150520015JH);吉林师范大学博士启动项目(2014004)

摘　　要：开发了一种共振拉曼光谱(RR)技术用于二价铜离子的定量评价的新方法.通常二价铜离子(Cu^(2+))在碱性的条件下能够被蛋白质还原成一价铜离子,而两个BCA探针分子能够与一价铜离子发生螯合反应,形成BCA/Cu^+复合物,它有很强的RR活性.因此,采用这一RR方法进行蛋白质检测,能够比传统方法的检测浓度范围更宽.并且,基于RR方法可以对Cu^(2+)的最低检测浓度达到5×10^(-7)mol/L,要比传统的比色和紫外可见吸收光谱法更灵敏.该方法提供了一种新颖且高度敏感的RR技术用于金属离子的定量研究.In this paper,we have proposed a rapid and highly sensitive Bicinchoninic Acid （BCA） reagent-based Cu2＋ ion quantitation tool using competitive resonance raman （RR） method.A chelation reaction between BCA and Cu＋ which is reduced by protein in an alkaline environment.Two BCA molecules complex with a Cu＋ create a BCA/Cu＋ complex that has strong RR activity.The proposed RR method exhibit a much wider linear concentration range compared to UV -based methods. The detection limits based on the proposed RR method is as low as 5 x 10-7 molJL , which is lower than the traditional method. This novel and sensitive method exhibited an excellent application in quantitative determination of metal ions.

关 键 词：共振拉曼 铜离子 检测 定量 

分 类 号：O657.37[理学—分析化学]