五参胶囊镇静催眠作用及其机制的实验研究  

Sedative and Hypnotic Effects and Mechanisms of Wushen Capsules

作　　者：柳旭 庞高宗 罗序华 丛鹤 夏钰晰 娄云婷 张艳雨 孙靖辉[1] 

机构地区：[1]北华大学药学院,吉林吉林132013 [2]北华大学第一临床医学院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2017年第5期594-597,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省科技发展计划项目(20150309004YY);国家级大学生创新创业训练计划项目(2013Q017);吉林市科技发展计划项目(201464062)

摘　　要：目的观察五参胶囊(Wu Shen Capsules,WSh C)对小鼠镇静催眠作用的影响,并探讨其作用机制.方法将ICR小鼠随机分为对照组、五参胶囊高剂量组、中剂量组和低剂量组.五参胶囊高剂量组用药量为200 mg/(kg·d^(-1)),中剂量组用药量为100 mg/(kg·d^(-1)),低剂量组用药量为50 mg/(kg·d^(-1)),给药时间为15 d,于给药结束后进行小鼠自主活动实验和协同戊巴比妥钠阈上剂量睡眠时间实验,测定5 min内各组小鼠的活动次数和站立次数,记录各组小鼠的睡眠潜伏期和睡眠持续时间.采用酶联免疫吸附法检测小鼠下丘脑5-羟色胺(5-Hydroxytryptamine,5-HT)、5-羟吲哚乙酸(5-Hydroxyindole acetic acid,5-HIAA)、γ-氨基丁酸(γ-aminobutyric acid,GABA)、谷氨酸(glutamine,Glu)含量,并计算5-HIAA/5-HT比值及Glu/GABA比值.结果与对照组比较,WSh C各给药组小鼠活动次数减少,睡眠潜伏期缩短,睡眠持续时间延长(P<0.01),WSh C能升高小鼠下丘脑5-HIAA含量和5-HIAA/5-HT比值,降低Glu含量和Glu/GABA比值.结论 WSh C具有明显镇静催眠作用,其机制可能与调节中枢兴奋性和抑制性神经递质有关.Objective To observe the sedative and hypnotic effects of Wushen Capsules （ WShC） and to explore the underlying mechanisms. Method Female ICR mice were randomly divided into 4 groups：control group,high-dose WShC group （200 mg/（kg·d-1））,medium-dose WShC group （100 mg/（kg·d-1））,and low-dose WShC group （50 mg/（ kg·d-1 ） ） ,and there were 12 mice in each group. All the mice in the different groups were given the corresponding agents orally and those in the control group were given the same volume of distilled water once a day continuously for 15 days. After the last administration,locomotor activities and standing times of mice in all the groups were measured by a multiple functional mice locomotor activities recorder, and the threshold dose pentobarbital sodium-induced sleep experiment was conducted, to examine the sedative and hypnotic effect of WShC. The contents of 5-Hydroxytryptamine （5-HT） ,5-Hydroxyindole acetic acid （5-HIAA） ,γ-aminobutyric acid （ GABA） and glutamine （ Glu） in the hypothalamus were detected with ELISA method for exploring the underlying mechanisms. Results Compared with the control group,the locomotor activity was reduced,the sleep latency was shortened and the sleep duration was prolonged in all the WShC-treated groups （ P〈0. 01 ）. WShC could significantly increase the contents of 5-HIAA and 5-HIAA/5-HT proportion;decrease the contents of Glu and Glu/GABA proportion in the hypothalamus. Conclusion WShC has a sedative and hypnotic effect on mice, which might be related to the regulation of central excitatory and inhibitory neurotransmitters.

关 键 词：五参胶囊 失眠 镇静催眠 神经递质 

分 类 号：R285[医药卫生—中药学]