仙茅组培快繁技术研究  

Study on Rapid Propagation via Tissue Culture of Curculigo

作　　者：张虹[1] 何钢[1] 刘贤桂[2] 梁文斌[1] 曾柏全[1] 

机构地区：[1]中南林业科技大学生命科学与技术学院,长沙410000 [2]中南林业科技大学林学院,长沙410000

出　　处：《特产研究》2017年第4期6-12,共7页Special Wild Economic Animal and Plant Research

基　　金：国家林业公益性行业科技专项项目(201304807)

摘　　要：以仙茅幼叶为材料,采用不同的激素诱导出愈伤组织,进而产生不定芽,不定芽继代产生小植株,小植株生根后移栽入土成活,从而建立了仙茅快繁技术。结果表明,MS+2,4-二氯苯乙酸(2,4-D)0.1mg/L+KT1.5mg/L+多菌灵3%+柠檬酸8mg/L诱导愈伤组织效果最好,可达96%。继代培养基为MS+NAA0.3mg/L+6-BA2mg/L+2,4-D0.3mg/L,继代效果最好、增殖率最高、愈伤组织的质量最好,较易诱导出不定芽。在培养基中添加0.3mg/L的NNA有利于生根,移栽至营养土中的成活率可达到90%以上。Callus was induced by different hormone concentrations in young leaves of Curculigo orchiodes.The suitable medium was se- lected to induce adventitious buds, and then the rooting experiment was carried out and transplanted to soil.The results showed that MS＋2,4-D0.1 mg/L＋KT1.Smg/L＋carbendazim 3%＋citric acid 8mg/L was the best in MS medium.Up to 96%.The subculture medium was MS＋NAA0.3mg/L＋6-BA2mg/L＋2,4-D0.3mg/L, the best subculture was the MS, the highest callus quality was the best, More easily induced adventitious buds.Adding 0.3mg/L NNA to the medium was beneficial to rooting, and the survival rate was 90% when transplan- ted into the nutrient soil.

关 键 词：仙茅 组织培养 不定芽 移栽 

分 类 号：S796[农业科学—林木遗传育种]