Investigation of Extracts from Tunisian Ethnomedicinal Plants as Antioxidants,Cytotoxins,and Antimicrobials  

作　　者：Guesmi Fatma Ben Hadj Ahmed Sami Landoulsi Ahmed 

机构地区：[1]Laboratory of Biochemistry and Molecular Biology,Faculty of Sciences of Bizerte,University of Carthage,Zarzouna 7021,Tunisia [2]Laboratory of Protein Engineering and Bioactive Molecules(LIP-MB),National Institute of Applied Sciences and Technology(INSA T),BP 676,1080 Tunis Cedex,Tunisia

出　　处：《Biomedical and Environmental Sciences》2017年第11期811-824,共14页生物医学与环境科学（英文版）

摘　　要：Objective To determine the medicinal potential of various plants and their parts extracted with different solvents. Methods The total phenolic content of acetonitrile/water（60%-40%）（ACN/W） and aqueous（W） extract fractions was determined by high-performance liquid chromatography（HPLC）, and terpenic compounds were detected by gas chromatography/mass spectrometry（GC/MS）. Antioxidant activity of the samples was evaluated using 2,2-diphenyl-1-picrylhydrazyl（DPPH） assay and β-carotene bleaching method. Cell viability was investigated by thiazolyl blue tetrazolium bromide [3-（4,5-dimethylthiazol）-2-yl 2,5-diphenyltetrazolium bromide]（MTT） assay. The mechanisms involved in cytotoxic activity were investigated in a murine macrophage cell line（RAW 264.7） and cancer lines. Results Our findings show that 11 plant species exhibited biological activity. In addition, moderate antibacterial activity was reported against one or more of the tested bacterial strains at two concentrations： 300 μg and 3 mg/disc. Furthermore, our data reveal that among all plants investigated, some extract and hydrophobic fractions were potent scavengers of the DPPH radical（6.78 μg/m L 〈 EC50 〈 8.55 μg/mL）. Taken together, our results show that Nerium oleander（NOACN/W） and Pituranthos tortuosus（PTACN/W） were highly cytotoxic against RAW 264.7 cells with IC80 values of 0.36, and 1.55 μg/mL, respectively. In contrast, murine macrophage cell lines had low growth and were significantly sensitive to water extracts of Thymus hirtus sp. algeriensis（THW）, Lavandula multifida（LMW）, and ACN/W extract of Erica multiflora（EMACN/W） at doses 〉 400, 47.20, and 116.74 μg/m L, respectively. The current work demonstrates that RAW 264.7 cell proliferation was inhibited by samples in a dose-dependent manner. Conclusion Our findings, validated through free radical scavenging activity, agar diffusion assay, and cytotoxicity of essential oils towards cancer cells, show that ethnomedicinal plants used Objective To determine the medicinal potential of various plants and their parts extracted with different solvents. Methods The total phenolic content of acetonitrile/water（60%-40%）（ACN/W） and aqueous（W） extract fractions was determined by high-performance liquid chromatography（HPLC）, and terpenic compounds were detected by gas chromatography/mass spectrometry（GC/MS）. Antioxidant activity of the samples was evaluated using 2,2-diphenyl-1-picrylhydrazyl（DPPH） assay and β-carotene bleaching method. Cell viability was investigated by thiazolyl blue tetrazolium bromide [3-（4,5-dimethylthiazol）-2-yl 2,5-diphenyltetrazolium bromide]（MTT） assay. The mechanisms involved in cytotoxic activity were investigated in a murine macrophage cell line（RAW 264.7） and cancer lines. Results Our findings show that 11 plant species exhibited biological activity. In addition, moderate antibacterial activity was reported against one or more of the tested bacterial strains at two concentrations： 300 μg and 3 mg/disc. Furthermore, our data reveal that among all plants investigated, some extract and hydrophobic fractions were potent scavengers of the DPPH radical（6.78 μg/m L 〈 EC50 〈 8.55 μg/mL）. Taken together, our results show that Nerium oleander（NOACN/W） and Pituranthos tortuosus（PTACN/W） were highly cytotoxic against RAW 264.7 cells with IC80 values of 0.36, and 1.55 μg/mL, respectively. In contrast, murine macrophage cell lines had low growth and were significantly sensitive to water extracts of Thymus hirtus sp. algeriensis（THW）, Lavandula multifida（LMW）, and ACN/W extract of Erica multiflora（EMACN/W） at doses 〉 400, 47.20, and 116.74 μg/m L, respectively. The current work demonstrates that RAW 264.7 cell proliferation was inhibited by samples in a dose-dependent manner. Conclusion Our findings, validated through free radical scavenging activity, agar diffusion assay, and cytotoxicity of essential oils towards cancer cells, show that ethnomedicinal plants used

关 键 词：Phenolic compounds CYTOTOXICITY ANTIOXIDANT ANTIBACTERIAL 

分 类 号：R284[医药卫生—中药学]