嘎日迪-13对大鼠脑缺血后不同时相不同脑区ICAM-1、VCAM-1表达的影响  

Effect of Garidi-13 on Expressions of ICAM-1 and VCAM-1 at Different Phase at Different Encephalic Region after Cerebral Ischemic in Rats

作　　者：曹丽霞[1] 郑延泽[1] 武海军[1] 何静波[1] 田彩云[1] 张浩楠[1] 覃建民[1] 徐继辉[1] 杨玉梅[1] 

机构地区：[1]包头医学院心血管研究室,内蒙古包头014040

出　　处：《中华中医药学刊》2017年第12期3009-3014,I0018,I0019,共8页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81160560)

摘　　要：目的:探讨脑组织中细胞间黏附分子-1(intercellular adhesion molecule-1,ICAM-1)和血管细胞黏附分子-1(vascular cell adhesion molecule-1,VCAM-1)在大鼠脑缺血损伤后不同时相不同脑区的表达及嘎日迪-13对其影响。方法:雄性SD大鼠360只,将大鼠随机分为假手术组、脑缺血模型组、嘎日迪-13大剂量组(240 mg/kg)、嘎日迪-13中剂量组(120 mg/kg)、嘎日迪-13小剂量组(60 mg/kg)5组,每组又随机分为缺血1h组、6 h组、12 h组、24 h组、72 h组与120 h组6个时相组,采用改良Zea Longa线栓法制备大鼠大脑中动脉栓塞模型,免疫组织化学法检测脑组织中ICAM-1和VCAM-1在1 h、6 h、12 h、24 h、72 h和120 h蛋白的表达。结果:与假手术组比较,模型组缺血侧海马、纹状体和大脑皮质区ICAM-1、VCAM-1阳性表达量显著升高,1 h后开始上升,24 h达高峰,之后逐渐下降(P<0.01),具有显著性差异。嘎日迪-13大、中、小剂量组缺血侧海马、纹状体和大脑皮质区可明显降低ICAM-1、VCAM-1的阳性表达量,与模型组比较具有显著性差异(P<0.01或P<0.05)。结论:嘎日迪-13可能是通过减少脑缺血损伤后各时相神经元的损伤,调节黏附分子相互之间的作用,抑制其合成、分泌及释放,来减轻脑组织损伤,从而发挥对脑缺血损伤的保护作用。Objective ： To investigate the expressions of intercellular adhesion molecule - 1 （ ICAM - 1 ） and vascular cell adhesion moleculel （ VCAM - 1 ） in brain tissue at different phase at different encephalic region after cerebral ische- mic in rats as well as the effect of Gridi - 13 on them. Methods ： A total of 360 male SD rats were selected. They were randomly divided into sham- operated group, model group, Gridi - 13 large dose group, Gridi - 13 middle dose group and Gridi - 13 small dose group. Each group was randomly divided into 1 h, 6 h, 12 h, 24 h, 72 h and 120 h time phase groups. The middle cerebral artery occlusion （MCAO） model was established by modified Zea Longa thread - occlusion method and the contents of ICAM - 1 and VCAM - 1 in brain tissue were determined by Immunohistochemical method. Results： Compared with the sham group, model group~ ischemic hippocampus, striatum and cerebral cortex positive ex- pressions of ICAM - 1 and VCAM - 1 were significantly increased, while rising after the cerebral ischemic injury for 1 h, reaching the peak at 24 h, and then going down gradually, with significant differences （P 〈0.01 ）. Garidi -13 in each dose group＇s ischemic hippocampus, striatum and cerebral cortex can significantly reduce the protein expression levels of ICAM - 1 and VCAM - 1, and had significant difference compared with those of the model group （ P 〈 0.01 or P 〈 0.05）. Conclusion： Gridi -13 may be related with alleviating neuronal damage, regulating adhesion molecule and relie- ving the injury of brain tissue to play a role of therapeutic action in cerebral ischemic injury.

关 键 词：嘎日迪-13 脑缺血 ICAM-1 VCAM-1 

分 类 号：R285.5[医药卫生—中药学]