β-细辛醚对Aβ_(1-42)诱导PC12细胞炎症因子表达的影响  

Experimental Study on Impact of β-Asarone on Inflammatory Cytokines Secreted by Pc12 Cells Induced by Aβ_(1-42)

作　　者：包玉婷[1] 何潆 周小杰[1] 戴世杰[1] 杨元宵[3] 

机构地区：[1]浙江中医药大学,浙江杭州310053 [2]杭州市红十字会医院,浙江杭州310003 [3]杭州医学院,浙江杭州310053

出　　处：《中华中医药学刊》2017年第12期3019-3023,I0020,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81403128);浙江省科学技术厅项目(2018C37081)

摘　　要：目的:探讨β-细辛醚对Aβ_(1-42)诱导PC12细胞炎症因子表达的影响。方法:(1)将对数生长期PC12细胞随机分为正常对照组、Aβ_(1-42)3.3μM、Aβ_(1-42)10μM、Aβ_(1-42)30μM。采用实时无标记动态细胞分析技术(Real Time Cell Analusis,RTCA)和MTT法检测各组PC12细胞存活率;Western blotting检测PC12细胞的IL-1β和TNF-α蛋白表达的变化。(2)将对数生长期PC12细胞随机分为正常对照组、Aβ_(1-42)模型组、β-细辛醚18.5μg·mL^(-1)、β-细辛醚55.5μg·mL^(-1)、β-细辛醚166.7μg·mL^(-1)。采用Western blotting检测PC12细胞的IL-1β和TNF-α蛋白表达的变化。结果:(1)3.3μM Aβ_(1-42)能显著增加PC12细胞IL-1β和TNF-α的蛋白表达(P<0.05),对PC12细胞的存活率无显著影响(P>0.05)。(2)与正常对照组相比,Aβ_(1-42)模型组细胞的IL-1β和TNF-α的表达明显增加(P<0.01)。与Aβ_(1-42)模型组相比,β-细辛醚(55.5μg·mL^(-1)、166.7μg·m L^-1)能显著降低PC12细胞IL-1β、TNF-α的表达(P<0.01),β-细辛醚(18.5μg·mL^(-1))能降低PC12细胞IL-1β的表达(P<0.05),但对TNF-α的表达无显著影响(P>0.05)。结论:β-细辛醚可以有效地抑制Aβ_(1-42)诱导的PC12细胞分泌IL-1β、TNF-α的蛋白表达。Objective ：To investigate the impact of β -asarone on inflammatory cytokines secreted by PC12 cells in- duced by Aβ1-42. Methods：（ 1 ）PC12 cells cultured in vivo were divided into 4 groups, namely control group, low dose Aβ1-42 group （3.3 μM）, middle dose Aβ1-42 group （10 μM）, high dose Aβ1-42 group （30 μM）. Real Time Cell Analusis （RTCA） and MTT were used to evaluate the survival rate of PC12 cells. The expressions of IL - 1β and TNF - α were examined by Western blotting. （2）PC12 cells cultured in vivo were divided into 5 groups, namely control group, model group, low dose β- asarone group （18. 5 μg/mL）, middle dose β -asarone group（55.5 μg/mL）, high dose β - asarone group （ 166. 7 μg/mL）. The expressions of IL - 1β and TNF -α were examined by Western blotting. Results ： （1） The Aβ1-42 at the dose of 3.3 μM could up regulate the expressions of IL- 1βand TNF- α（ P 〈 0.05 ）and had no significant effect on the survival rate of PC12 cells （ P 〉 0.05 ）. （ 2 ） The results showed that the expressions of IL - 1β and TNF - α were up regulated in model group compared with those of the control group（P 〈0. 01 ）. The β - asarone at the dose of 55.5 μg/mL and 166. 7 μg/mL could slow down the expressions of IL - 1β and TNF - α（P 〈0. 01 ）. The 13 - asarone at the dose of 18.5 μg/mL could slow down the expression of IL - 1β（ P 〈 0. 05 ） and had no significant effect on the expression of TNF - α （ P 〉 0. 05 ）. Conculusion ： The β - asarone could significantly inhibit the expressions of of IL - 1β and TNF -α in PC12 cells induced by Aβ1-42.

关 键 词：β-细辛 AΒ1-42 PC12细胞 RTCA IL-1Β TNF-α 

分 类 号：R285.5[医药卫生—中药学]