苍术药材的质量标准研究  

Quality Standard of Atractylodis Rhizoma

作　　者：朱利霞 黄青[1] 王茹静[1] 李鸿翔[1] 谭玉柱[1] 董小萍[1] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家实验室培育基地,四川成都611137 [2]恩施土家族苗族自治州民族医院,恩施土家族苗族自治州中心医院,湖北恩施445000

出　　处：《中华中医药学刊》2017年第12期3046-3051,I0021,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家基础科学培养基金项目(J13100340-11);四川省教育厅重点项目(15ZA0093);成都中医药大学科技发展基金项目(ZRQN1440)

摘　　要：目的:完善苍术药材的质量标准。方法:对10批茅苍术与10批北苍术药材,采用显微鉴别法、TLC法进行鉴别;并对其水分、杂质、总灰分、酸不溶性灰分、水溶性浸出物、醇溶性浸出物、重金属进行测定;采用HPLC法建立苍术药材的高效液相特征图谱及测定苍术素含量。结果:苍术药材粉末的显微鉴别与薄层鉴别特征显著。在高效液相特征图谱中,20批苍术检出4个主要共有特征峰,其相似度为0.93~0.98。苍术药材中苍术素含量测定在0.5~60 mg·L-1范围内具有良好的线性关系(R2=0.999 1,n=13),平均加样回收率茅苍术为97.84%(1.62%),北苍术为98.63%(RSD为0.81%)。结论:本研究优化后的苍术药材质控方法符合中药质量控制的整体性、综合性及可适用性的要求,可用于苍术药材质量的综合评价。Objective： To improve the quality standard of Atractylodis Rhizoma. Methods： Microscopic features were studied based on the powder of both 10 batches of Atractylodes lancea （ Thunb. ） DC. and Atractylodes chirtensis （ DC. ） Koidz.. Water, foreign matter, total ash, acid insoluble ash, water - soluble extractive, 70% ethanol - soluble extrac- tive and heavy metals were all determined. The characteristic chromatogram of Atractylodis Rhizoma was established and the content of atractylodin was assayed all by HPLC. Results ： Microscopic features of the powder and TLC identification of Atraetylodis Rhizoma were remarkable. Four peaks were clearly identified as main characteristic peaks in HPLC ehromat- ograms of 20 batches of Atractylodis Rhizoma, and their similarities were 0. 93 - 0. 98. The assay method of atractylodin showed that it was in a good linear range of 0. 5 -60 mg L-1（R2 =0. 999 1, n = 13）. The average recovery forAtrac- tylodes lancea （ Thunb. ） DC. and Atractylodes chinensis （ DC. ） Koidz. was 97.84% （ RSD = 1.62% ）, 98.63% （ RSD = 0. 81% ）, respectively. Conclusion： This optimized quality control method can meet the requirement of integrity, com- prehesiveness and applicability in quality control of traditional Chinese medicine. Therefore, the established method can be used to evaluate the comprehensive quality of Atractylodis Rhizoma.

关 键 词：茅苍术 北苍术 苍术素 质量标准 显微鉴别 薄层色谱 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]