灸法对阿尔茨海默病小鼠胶质细胞相关蛋白表达的影响  

Effect of Grain-sized Moxibustion on Related Proteins of Glial Cells in Alzheimer's Disease Transgenic Mice

作　　者：余静[1] 包烨华[1] 楚佳梅[1] 高灵爱[2] 叶承锋 

机构地区：[1]杭州市中医院针灸科,浙江杭州310007 [2]杭州市余杭区第五人民医院,浙江杭州310000 [3]桐庐县中医院骨科,浙江桐庐311500

出　　处：《中华中医药学刊》2017年第12期3149-3153,I0035,I0036,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金项目(LY13H270002)

摘　　要：目的:探讨胶质纤维酸性蛋白(GFAP)、离子钙接头蛋白(Iba-1)在阿尔茨海默病(Alzheimer's disease,AD)发病中的作用,以及心俞、肾俞麦粒灸法对其的影响。方法:鉴定APP/PS1双转基因AD传代小鼠的基因表型,选取1.5月龄雌性转基因阳性Tg6799小鼠随机分为模型组9只和治疗组8只,同龄、同背景的C57BL/6J野生型雌性小鼠9只为正常对照组。治疗组取双侧心俞、肾俞行麦粒灸治疗,每日1次,10次为1个疗程,共治疗9个疗程,每个疗程之间间隔2 d。应用免疫组化法检测小鼠额叶皮层及海马区GFAP、Iba-1的表达水平。结果:与正常组相比,模型组额叶皮层区和海马区GFAP、Iba-1的表达增强(P<0.01);经麦粒灸法治疗后,治疗组小鼠大脑额叶皮层和海马区GFAP、Iba-1表达较模型组减少(P<0.05)。结论:心俞、肾俞麦粒灸疗法可减少GFAP、Iba-1的表达,抑制AD病理过程中胶质细胞的过度激活、增生,抑制神经炎症反应。Objective ： To observe the effect of grain - sized moxibustion intervention on expresion of glial fibrillary a- cidic protein and ionized calcium - binding adaptor molecule 1 in the area of cortex and hippocampus in Alzheimer＇ s dis- ease （AD） rats so as to explore its mechanisms underlying improvement of AD in clinic. Methods： The genotyping of transgenic mice was detected by PCR. The 1.5 months transgenic （Tg） 6799 mice were randomly divided into model group（ n = 9） and treatment group （n = 8）. Normal group adopted the same age and background C57BL/6J female mice （n = 9 ）. Moxibustion （ ignited grain - sized moxa cone） was applied to bilateral ＂Xinshu＂ （ BL15 ） and ＂Shenshu＂ （BL23） once a day for nine courses（ 10 days constitute a therapeutic course） only in treatment group. GFAP and Iba- 1 （positive area, integrate optical density） in the area of cortex and hippocampus were detected by immunohistochemical- ly method. Results ： In comparison with the normal group, the expression levels of GFAP and Iba - 1 in the area of cortex and hippocampus were upregulated significantly in the model group （P 〈 0. O1 ）. After 9 sessions of grain - sized moxi- bustion intervention, the expression levels of GFAP and Iba- 1 were down- regulated significantly（P 〈 O. 05 ）. Conclu- sion： Grain- sized moxibustion at ＂Xinshu＂ （BL15） and ＂Shenshu＂ （BL23） can reduce the expressions of GFAP and Iba - 1 in the area of cortex and hippocampus, suggesting an improvement of AD after moxibustion intervention by restrai- ning the inflammatory reaction.

关 键 词：APP PS1转基因鼠 胶质纤维酸性蛋白 离子钙接头蛋白 麦粒灸 

分 类 号：R245.8[医药卫生—针灸推拿学]