百合知母汤对CUMS抑郁症大鼠海马中ERK^(1/2)信号通路关键分子的影响  

Influence of Baihe Zhimu Decoction in Leveles of Key Factors of ERK^(1/2) Singaling Pathway in Hippocapus Tissues of Depression Rats

作　　者：曹秋实[1] 李德顺[1] 袁丽[1] 吴建红[1] 韩永明[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《中华中医药学刊》2017年第12期3154-3157,I0037,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：湖北省教育厅科研计划重点项目(D20142001)

摘　　要：目的:研究百合知母汤对CUMS抑郁症大鼠ERK^(1/2)信号通路中的关键分子的影响。方法:SPF级SD大鼠50只,随机分为空白组、氟西汀组、百合知母汤中剂量组、百合知母汤大剂量组、模型组,每组10只。除空白组外,其余大鼠CUMS法制造抑郁症大鼠模型,共计21 d;造模完毕后,各组分别给予氟西汀(1.8 mg/kg)、百合知母汤(1.5 mg/kg)、百合知母汤(3.0 mg/kg),空白组、模型组均给予等体积生理盐水,共计28 d;给药完毕后,取大鼠海马组织,分别用Western-blot法、q PCR法、免疫组化法检测PKC蛋白、MEKmRNA、ERK^(1/2)蛋白的表达量。结果:与模型组比较,百合知母汤中、高剂量组海马组织中的PKC蛋白、MEKmRNA、ERK^(1/2)蛋白的表达量均升高(P<0.05或P<0.01)。结论:百合知母汤的抗抑郁机制可能与上调CUMS抑郁症大鼠海马组织中ERK^(1/2)信号通路的关键分子PKC蛋白、MEKmRNA及ERK^(1/2)蛋白有关。Objective： To study the influence of Baihe Zhimu Decoction （BZD） in leveles of key factors of ERK1/2 sin- galing pathway in hippocapus tissues of depression rats. Methods： Fifty SD rats of class SPF were divided into control group, model group, moderate and high doses of BZD group and fluoxetine group （n = 10）. Expect for rats of control group, all the rats in the other groups were made depression models by chronic unpredictable mild stress （CUMS）, total- ly 21 days. From the 22 nd day, all the rats were fed with NS, NS, BZD （ 1.5 mg/kg, 3.0 mg/kg） and fluoxetine （ 1.8 mg/kg） expectively for 28 days. The levels of protein kinase C （PKC） protein, mitogen extracellular kinaes（MEK） mR- NA and extracellular signal regulated protein kinase （ ERK1/2 ） protein were tested by means of Western - blot, qPCR and immunohisto - chemistry correspondingly. Results ： Contrast to the model group, the levels of PKC protein, MEKmRNA and ERK1/2 protein in the hippocapus tissues of moderate and high doses group rats were increased after the treatment with BZD statistical significance （P 〈 0. 05 or P 〈 0.01 ）. Conclusion： The antidepressant mechanism of BDZ is related to its heightening the PKC protein, MEKmRNA and ERK1/2 protein in the hippocapus tissues of depression rats.

关 键 词：抑郁症 百合知母汤 蛋白激酶C 细胞外信号调节蛋白激酶 丝裂原细胞外激酶 

分 类 号：R285.5[医药卫生—中药学]