脑络欣通对大脑中动脉阻塞再灌注模型大鼠神经干细胞增殖分化的影响  

Effect of Naoluoxintong on Proliferation and Differentiation of Neural Stem Cells in a Rat Model of Middle Cerebral Artery Occlusion/Reperfusion

作　　者：韩义皇[1] 王键[2] 

机构地区：[1]北京中医药大学,北京100029 [2]安徽中医药大学,安徽合肥230012

出　　处：《安徽中医药大学学报》2017年第6期58-63,共6页Journal of Anhui University of Chinese Medicine

基　　金：国家科技支撑计划项目(2011BA03159)

摘　　要：目的观察脑络欣通对大脑中动脉阻塞再灌注(middle cerebral artery occlusion-reperfusion,MCAO-R)模型大鼠神经干细胞增殖分化的影响。方法采用线栓法复制MCAO-R大鼠模型,将30只健康雄性SD大鼠随机分成假手术组、模型组和给药组。分别采用神经功能评分和TTC染色鉴定模型大鼠的神经功能缺损和脑缺血面积,采用免疫荧光染色法观察大鼠海马CA1、CA2、CA3、齿状回(dentate gyrus,DG)区巢蛋白(Nestin)和神经丝蛋白-H(hypophosphorylated neurofilament-H,NF-H)表达水平。结果给药组Nestin阳性表达主要发生在DG区,NF-H阳性表达主要发生在CA3区。脑络欣通促进神经干细胞增殖的作用在DG、CA1、CA2、CA3区均十分明显,以DG区尤为突出,促进神经干细胞分化的作用主要表现在CA3、DG、CA2、CA1区。与模型组比较,给药组大鼠海马各区的Nestin、NF-H阳性细胞均明显增多,差异具有统计学意义(P<0.05)。结论脑络欣通具有促进海马区神经干细胞增殖并分化为神经细胞的作用。Objective To investigate the effect of Naoluoxintong on the proliferation and differentiation of neural stem cells in a rat model of middle cerebral artery occlusion/reperfusion （MCAO/R）. Methods The suture method was used to establish a rat model of MCAO/R, and a total of 30 healthy male Sprague-Dawley rats were randomly divided into sham-operation group, model group, and administration group. The neurologi- cal score was used to evaluate neurological defects, TTC staining was used to measure the area of cerebral ischemia, and immunofluorescent staining was used to observe the expression of Nestin and hypophospho- rylated neurofilament-H （NF-H） in the CA1, CA2, CA3, and dentate gyrus （DG） regions of the hippo- campus. Results In the administration group, Nestin was mainly expressed in the DG of the hippocampus and NF-H was mainly expressed in the CA3 region of the hippocampus. Naoluoxintong significantly pro- moted the proliferation of neural stem cells in the CA1, CA2, CA3, and DG regions of the hippoeampus, especially in the DG region, and it significantly promoted the differentiation of neural stem cells in the CA3, DG, CA2, and CA1 regions. Compared with the model group, the administration group had signifi- cant increases in the numbers of cells with positive Nestin and NF-H in each region of the hippoeampus （P〈0.05）. Conclusion Naoluoxintong can promote the proliferation of neural stem cells and the differen- tiation of such ceils into neural cells in the hippoeampus.

关 键 词：脑络欣通 大脑中动脉阻塞再灌注模型 海马 神经干细胞 增殖分化 免疫荧光染色 

分 类 号：R285.5[医药卫生—中药学] R743.3[医药卫生—中医学]