金银花体外抑制乙酰胆碱酯酶活性部位筛选及液质联用分析研究  

Investigation Hypoglycemic and Antioxidant Activities of the Refined Extract of Glycyrrhiza uralensis in vitro and Analysis of it by HPLC-DAD-ESI-MS/MS

作　　者：赵娜娜[1,2] 孟令文[2] 孙道磊[3] 杨银平 姜婧[2] 张辉 孙佳明[2] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]长春中医药大学,吉林长春130117 [3]云南白药集团中药资源有限公司,云南昆明650100

出　　处：《时珍国医国药》2017年第10期2358-2360,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然基金面上项目(31570347);吉林省卫生和计划生育委员会科研计划课题(2015Z067)

摘　　要：目的筛选金银花体外抑制乙酰胆碱酯酶的活性部位,并对其化学成分进行分析。方法金银花提取物经反相C_(18)填料进行固相萃取(SPE)制备不同浓度甲醇梯度洗脱部位,以体外抑制乙酰胆碱酯酶能力评价其抗阿尔兹海默症作用,采用高效液相色谱-质谱联用技术对金银花化学成分进行分析。结果金银花提取物的30%甲醇洗脱部位的乙酰胆碱酯酶酶抑制活性明显强于其他洗脱部位,并存在明显的量效关系,为其体外抑制乙酰胆碱酯酶的活性部位,经液质联用分析共鉴定出14种化合物成分。结论金银花具有体外抑制乙酰胆碱酯酶活性,其主要有效部位为30%甲醇洗脱部位,具有一定的抗阿尔兹海默症的作用。Objective Screening honeysuckle in vitro inhibition of acetylcholinesterase active site,and to analyze the chemical composition. Methods The method of honeysuckle extract by reverse phase C_（18） material subjected to solid phase extraction（ SPE） was prepared with different concentrations of methanol alcohol gradient site,in vitro inhibition of acetylcholinesterase ability to evaluate the effect of anti-Alzheimer＇s disease,high performance liquid chromatography-mass spectrometry coupled with honeysuckle chemical composition analysis. Results Honeysuckle extract water will result in 30% methanol site acetylcholinesterase inhibitory activity was significantly stronger than other parts of the elution,and there is a clear dose-effect relationship for the honeysuckle in vitro inhibition of acetylcholinesterase active site,the LC-MS analysis identified a total of 14 phenolic acids.Conclusion Honeysuckle in vitro inhibition of acetylcholinesterase activity,its main active ingredient chlorogenic acid represented by phenolic acids,has a certain anti-Alzheimer＇s effect.

关 键 词：金银花 乙酰胆碱酯酶 阿尔兹海默症 HPLC-MS 

分 类 号：R284.2[医药卫生—中药学]