葛根中大豆苷的高速离心分配色谱法分离纯化  

Isolation and Purification of Daidzin from Pueraria lobata by Fast Centrifugal Partition Chromatography

作　　者：谢欣辛 朱全刚 陈中建 孙百玲 杨义芳 

机构地区：[1]上海市皮肤病医院,上海200443 [2]中国医药工业研究总院中药创新中心,上海201203

出　　处：《时珍国医国药》2017年第10期2386-2388,共3页Lishizhen Medicine and Materia Medica Research

基　　金：上海市国际合作项目(12120701400);上海市自然科学基金(09ZR1431100);国家"重大新药创制"科技重大专项(2009ZX09301-007);上海市中药现代化专项资助项目(10DZ1971300);上海市科委西部开发科技合作项目(10495801000)

摘　　要：目的应用高速离心分配色谱法(FCPC)分离纯化葛根中的大豆苷。方法以乙酸乙酯-正丁醇-乙醇-水(5:1:0.5:5)作为两相溶剂系统,上相为流动相,下相为固定相,转速1400 r/min,流速3.0 m L/min,检测波长254 nm,采用ascending模式进行分离制备,化合物的结构由质谱及核磁共振谱鉴定。结果从1.0 g葛根粗提物中分离得到16.3 mg大豆苷,纯度经HPLC分析为96.5%。结论该方法适合于葛根中的大豆苷的高效分离制备。Objective To isolate and purify daidzin from the roots of Pueraria lobata by fast centrifugal partition chromatography（ FCPC）. Methods The experiment was performed with a two phase solvent system composed of ethyl acetate-n-butanol-ethanol-water（ 5： 1： 0. 5： 5,v/v）. The upper phase as mobile phase in ascending mode was operated at a rotation speed of 1400 r/min and the flow rate of 3. 0 m L/min with the detection wave length at 254 nm. The compound was analyzed by high performance liquid chromatography（ HPLC）,and elucidated by comprehensive NMR and ESI-MS analysis. Results A total 16. 3 mg of daidzin with the purity about 96. 5% was successfully obtained by FCPC from 1. 0 g of the crude extract of Pueraria lobata. Conclusion The FCPC was a simple,robust and efficient method for the preparation of daidzin from Pueraria lobata.

关 键 词：高速离心分配色谱 葛根 大豆苷 

分 类 号：R284.1[医药卫生—中药学]