云南省急性弛缓性麻痹病例中柯萨奇病毒B5VP1区基因特征分析  

Genetic characteristics of Coxsackievirus B5 isolated from acute flaccid paralysis patients in Yunnan province

作　　者：陈苏 张杰[2] 田炳均[2] 汤晶晶[2] 李凯[2] 李琼芬[2] 丁峥嵘[2] 陆林[2] 

机构地区：[1]昆明医科大学公共卫生学院,云南昆明650000 [2]云南省疾病预防控制中心

出　　处：《中国公共卫生》2017年第12期1745-1748,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81160198)

摘　　要：目的分析云南省2015年从急性弛缓性麻痹(AFP)病例中分离到的3株柯萨奇病毒B5(CVB5)VP1区核苷酸基因特征。方法采集RD细胞和L20B细胞对AFP标本进行病毒分离,应用反转录-聚合酶链反应(RT-PCR)扩增病毒VP1基因,目的基因送至基因公司进行序列测定;应用MEGA 6软件对分离到的病毒与基因库代表株进行进化树分析。结果 213例AFP标本中分离到23株非脊髓灰质炎肠道病毒(NPEV),包括3株CVB5;3株CVB5与原型株VP1区之间核苷酸(nt)相似性为80.7%~81.9%,氨基酸(aa)相似性为95.1%~96.5%;系统进化树结果显示,本研究分离的CVB5与山东分离株(JN712675)的nt相似性为91.7%~92%,与安徽分离株(KF751191)的nt相似性为92%~93%。结论云南省2015年从AFP病例中分离到的3株CVB5与山东代表株(JN712675)和安徽代表株(KF751191)相似性较高。Objective To analyze genetic features of VP1 region of three Coxsackievirus B5 （CVB5） strains isolated from acute flaccid paralysis （AFP） patients in Yunnan province,2015. Methods Human rhabdomyosarcoma （RD） cells and L20B cells were used for virus isolation. Reverse transcriptase polymerase chain reaction （RT-PCR） was adopted for the amplification of VP1 region of isolated CVB5 strains; then the amplificated genes were sent to Gene Company for se- quencing fo target genes. The phylogenetic tree for the isolated strains and its representatives in gene bank were made with MEGA 6 software. Neighbour-joining method,kimura-2-parameter and 1 000 pseudo replicate datasets were used to construct the phylogenetic trees. Results Totally 23 non-polio enteroviruses （NPEV） strains were isolated from 213 AFP cases, including 3 CVB5 strains. The similarity of nucleotide of VP1 region between CVB5 isolates and its represent- atives was 80. 7 % -81.9%, while that of amino acid was 95. 1% -96. 5 %. Phylogenetic trees analysis showed that the similarity between nucleotide of CVB5 isolates and that of Shandong isolate （JN712675） and Anhui isolate （KF751191 ） were 91.7% -92% and 92% -93% ,respectively. Conclusion The 3 CVB5 strains isolated from AFP cases in Yunnan province are of high similarity to the Shandong isolate （JN712675） and Anhui isolate （KF751191）.

关 键 词：柯萨奇病毒B5(CVB5) VP1区基因 特征 急性弛缓性麻痹(AFP) 

分 类 号：R373.2[医药卫生—病原生物学]