β-谷甾醇对H_(22)荷瘤小鼠体内抗肿瘤作用  被引量:54

Anti-tumor effect of β-sitosterol in H_(22) tumor-bearing mice

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作  者:林明珠 赵岩[1] 蔡恩博[1] 祝洪艳[1] 郜玉钢[1] 何忠梅[1] 杨鹤[1] 刘双利[1] 张连学[1] 

机构地区:[1]吉林农业大学中药材学院,吉林长春130118

出  处:《中国公共卫生》2017年第12期1797-1800,共4页Chinese Journal of Public Health

基  金:国家公益性行业科研专项(201303111);吉林省科技发展计划项目(20140204013YY;20150307012YY)

摘  要:目的探讨β-谷甾醇对H_(22)荷瘤小鼠体内抗肿瘤作用及其机制。方法建立H_(22)荷瘤小鼠模型,将荷瘤小鼠随机分为模型组、环磷酰胺组、β-谷甾醇高、低剂量组,每组10只,连续给药14 d,眼球取血后颈椎脱臼处死,称取H_(22)荷瘤小鼠的实体瘤重,计算抑瘤率、脾指数、胸腺指数,测定血清学指标,并进行肿瘤和脾脏组织病理学观察。结果与模型组比较,环磷酰胺组、β-谷甾醇高、低剂量组小鼠瘤重减轻,差异均有统计学意义(P<0.05),环磷酰胺组、β-谷甾醇高、低剂量组小鼠抑瘤率分别为56.1%、53.0%、41.4%;与对照组比较,模型组小鼠血清中白细胞介素-6(IL-6)、血管内皮生长因子(VEGF)水平[分别为(1 112.47±76.15)、(512.75±22.55)pg/mL]明显升高(P<0.05);与模型组比较,高剂量β-谷甾醇组小鼠血清中IL-6、VEGF水平[分别为(341.31±31.19)、(356.92±19.45)pg/mL]明显下降,而IFN-γ水平[(122.92±13.66)pg/mL]明显升高(P<0.05);与模型组比较,β-谷甾醇组小鼠肿瘤组织细胞出现一定的坏死区域,肿瘤组织细胞凋亡明显。结论β-谷甾醇对H_(22)荷瘤小鼠具有一定的抑瘤作用,其机制可能与其对IL-6、IFN-γ和VEGF表达的调节有关。Objective To study antitumor effect of β-sitosterol and its mechanism in H22 tumor-beating mice. Methods The tumor-bearing ICR mouse model was established with intraperitoneal injection of H22 cell suspension and the 10 mice with intraperitoneal injection of normal saline were used as the controls. Then the model mice were randomly divided into a model group, a cyclophosphamide (30 mg/kg) group, and low- and high-dose β-sitosterol (10 and 50 mg/kg) groups (10 mice in each group). All the treatments were administered through intraperitoneal injection once a day continuously for 14 days. At the end of the treatments ,the weight of tumor tissues from the tumor-bearing mice was determined and the tumor inhibition rate, the spleen index and the thymus index of the mice were calculated ;in addition, serological oarameters were measured and the histooatholoical chanes in organs of the mice were observed. ResultsCompared with that of the model group,the weight of tumor tissues was significantly lower in the mice of cyclophospha- mide and low- and high-dose [3-sitosterol group (P 〈0. 05 for a11) ;the tumor inhibition rates were 56. 1% in the mice of cyclophosphamide group and 53.0% and 41.4% in the mice of low- and high-dose 13-sitosterol group. The serum levels of interleukin-6 ( IL-6, 1112.47 ± 76. 15 pg/mL) and vascular endothelial growth factor ( VEGF, 512.75 ± 22. 55 pg/mL) of the model group were significantly increased compared with those of the controls ( both P 〈 0.05 ). Compared with that of the model group,the serum levels of IL-6 (341.31 ± 31.19 pg/mL) and VEGF (356. 92 ± 19. 45 pg/mL) of the high-dose of β-sitosterol group were significantly decreased, while the level of interferon-~/ (IFN-~/, 122. 92 - 13.66 pg/mL) was significantly increased ( P 〈 0. 05 ). Necrosis of tumor tissues and obviously more apoptosis of tumor cells compared to that of the model group were observed in the mice of the β-sitosterol groups. Conclusion β-sitosterol exhibits a c

关 键 词:Β-谷甾醇 H(22)荷瘤小鼠 体内 抗肿瘤 

分 类 号:R114[医药卫生—卫生毒理学]

 

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