加碱对猪场废水厌氧消化液好氧处理过程酸化改进作用及其对菌群结构的影响  

Alleviating Acidification of Aerobic Post-treatment of Digested Piggery Wastewater by Adding Alkali and Its Influence on Microbial Community

作　　者：王伸[1,2] 邓良伟 姜奕圻[1,2] 王霜 徐则 郑丹[1,2] 

机构地区：[1]农业部沼气科学研究所,成都610041 [2]农业部农村可再生能源开发利用重点实验室,成都610041

出　　处：《中国沼气》2017年第6期3-11,共9页China Biogas

基　　金：国家生猪技术产业体系(CARS-36-10B);国家自然科学基金(31572450)

摘　　要：文章采用序批式活性污泥反应器(SBR),探讨了加碱(Na_2CO_3)对猪场废水厌氧消化液好氧处理酸化的改进作用及其对污染物去除的影响。在运行203天观察结果表明,混合液pH值从未加碱的5.7增长到加碱的7.9,NH_4^+-N去除率从76.8%增长到99.0%,但对COD,TIN和TP去除效果提高不明显。使用Hiseq高通量测序技术分析了污泥中微生物多样性的变化。研究结果表明:与SBR直接处理猪场废水厌氧消化液的对照(CG)组相比,加碱(AA)组富集的氨氧化细菌(AOB)(Nitrosomonas)的相对丰度远远大于亚硝酸盐氧化菌(NOB)(Nitrospira);可从微生物群落上解释加碱(AA)出现亚硝酸积累的原因。In this paper,sequencing batch reactor( SBR) was used,and alleviating acidification of aerobic post-treatment of piggery wastewater digested effluent was investigated by adding of alkali( Na_2CO_3). A 203-days observation showed that,after adding the Na_2CO_3,the pH of the reaction liquid increased from 5. 7 to 7. 8 in the reactor. And the removal efficiencies of NH_4^+-N increased from 76. 8% to 99. 0%. However,the removal efficiencies of COD,TIN and TP not increased significantly. High throughput sequencing technology of Hiseq was employed to analyze its influence on microbial community. The results showed that,comparing with the control group( CG),the relative abundance of enriched ammonia oxidizing bacteria( AOB)( Nitrosomonas) of alkali adding group( AA) was much higher than nitrite oxidizing bacteria( NOB)( Nitrospira). It is the reason that NO_2^--N was accumulated in AA group; there was little accumulation of NO_2^--N in control group.

关 键 词：猪场废水厌氧消化液 SBR 短程硝化 pH值 加碱 

分 类 号：S216.4[农业科学—农业机械化工程] X703[农业科学—农业工程]