Plant regeneration from protoplasts of hydroxyprolineresistant cell line in Onobrychis viciaefolia  

作　　者：XUZIQIN JINGFENJIA 

机构地区：[1]CellBiologyLaboratory,LanzhouUniversity,Lanzhou730000,CHina [2]Correspondingauthor

出　　处：《Cell Research》1995年第2期187-195,共9页细胞研究（英文版）

摘　　要：An efficient protocol for plant regeneration from protoplasts of hydroxyproline(HYP)resistant cell line of Onoblychis viciaefolia was established. In SH medium supplemented with 1 mg/L 2, 4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/L kinetin (KT) and 0.2 mg/L naphthalene acetic acid (NAA), the division frequency of protoplastderived cells reached uP to over 60 %, and microcalli were obtained in 5-6 wk. Upon transferring them on agar solidified MS medium plus 2 mg/L indole-3-acetic acid (IAA), shoots were induced. After cultivating them on MS medium with or without IAA, roots were regenerated.Chromosome number of all protoplast-regenerated plants examined were normal (2n=28). The protoplast-derived calli and plants grew vigorously on the medium containing 10 mmol/L HYP.

关 键 词：Sainfoin (Onobrychis Viciaefolia) hydroxyproline resistant cell line protoplast culture plant regeneration 

分 类 号：Q943.1[生物学—植物学] S541.4[农业科学—作物学]