职业性氟接触人群氟负荷与血清中可溶性Sema4D水平的相关性研究  

Serum soluble semaphorin4D levels in aluminum potroom workers exposed to fluoride emissions

作　　者：涂俊[1] 刘克俭[2] 旷聃[1] 金彦 刘富强[2] 张裕曾[2] 刘芸[2] 

机构地区：[1]成都市疾病预防控制中心,四川成都610041 [2]华中科技大学同济医学院公共卫生学院,湖北武汉430030 [3]华中科技大学同济医学院附属同济医院,湖北武汉430030

出　　处：《工业卫生与职业病》2017年第6期438-441,共4页Industrial Health and Occupational Diseases

基　　金：国家自然科学基金资助项目(81372941)

摘　　要：目的检测职业性氟接触人群血清中可溶性Sema4D(sSema4D)水平,探讨sSema4D与氟负荷的相关性。方法选取湖北省某铝业公司91名氟接触工人和54名行政职员为研究对象。应用问卷法调查其人口统计学特征。采用离子选择电极法测定尿氟。采用酶联免疫吸附试验(ELISA)法检测sSema4D水平。应用SPSS 17.0软件对数据进行统计分析。结果氟接触工人无氟性骨损伤组(APW-FBI)的尿氟(UF)水平(4.83±2.62 mg/L)高于对照组(1.30±0.11 mg/L)(P<0.01),氟接触工人有氟性骨损伤组(APW+FBI)的UF水平(15.0±3.35 mg/L)高于APW-FBI组(P<0.01);APW-FBI组的sSema4D水平(2.99±0.30 ng/mL)低于对照组(5.84±0.06 ng/mL)(P<0.01),APW+FBI组的sSema4D水平(1.90±0.24 ng/mL)低于APW-FBI组(P<0.01);UF与sSema4D呈负相关(r=-0.76,P<0.01)。结论氟接触工人(尤其氟性骨损伤)sSema4D水平较低,且与UF水平呈负相关。Objective To evaluate the serum soluble semaphoring 4 D（sSema4 D） in aluminum potroom workers, and to investigate the correlation between sSema4 D and fluoride burden. Methods A total of 91 potroom workers was studied,and the subjects were subdivided into aluminum potroom workers without Fluoride（F）bone injury（APW-FBI）and aluminum potroom workers with F bone injury（ APW ＋FBI）groups,respectively. Another 54 office workers were enrolled as the control group. Demographic characteristics were investigated using a questionnaire. Serum levels of sSema4 D were measured in all subjects using the enzymelabeled immunosorbent assay method. The UF levels was evaluated using ion selective electrode method. Data analysis was performed using SPSS 17. 0. Results The Urinary fluoride（UF） level in potroom workers was significantly increased compared with the control group（4. 83±2. 62 mg/L and 15. 0 ± 3. 35 mg/L vs. 1. 30±0.11 mg/L,P〈0.01）. UF concentrations were elevated in APW±FBI compared to APW-FBI（ 15. 0 ±3. 35 mg/L vs. 4. 83 ± 2. 62 mg/L, P〈0. 01）. The sSema4 D level in potroom workers was significantly decreased compared with the control group（1. 90±0. 24 ng/ml and 2. 99±0. 30 ng/ml vs. 5. 84±0. 06 ng/ml,P〈0. 01）. sSema4 D level was decreased in APW＋FBI compared to APW-FBI（1. 90±0. 24 ng/ml vs. 2. 99±0.30 ng/ml, P 0. 01）. Serum sSema4 D was negatively associated with UF（ r= 0. 76, P〈0. 01）.Conclusions Serum sSema4 D levels decrease in aluminum potroom workers（ especially in workers with F bone injury）and are associated with UF levels.

关 键 词：骨 氟 损伤 Sema4D 

分 类 号：R135.1[医药卫生—劳动卫生]