维生素E对PM2.5急性染毒致大鼠心血管损伤的保护作用  

Protective effects of Vitamin E on cardiovascular damage in rats caused by ambient PM_(2.5) acute infections

作　　者：李莉珊 马琼锦 刘洁 蒋蓉芳[2] 宋伟民[2] 

机构地区：[1]上海市闵行区疾病预防控制中心,上海201101 [2]复旦大学公共卫生学院公共卫生安全教育部重点实验室,上海200032

出　　处：《上海预防医学》2017年第11期835-841,共7页Shanghai Journal of Preventive Medicine

摘　　要：目的探讨维生素E(VE)对PM_(2.5)急性染毒致大鼠心血管损伤的干预作用。方法将36只雄性SD大鼠随机分为玉米油对照组(溶剂对照组)、VE对照组、PM_(2.5)染毒组(8.0 mg/kg,以体重计,下同)、PM_(2.5)+VE低、中、高给药组,剂量分别为15.0、30.0、60.0 mg/kg。VE给药组均经VE灌胃28 d后,气管滴注PM_(2.5)悬浊液染毒,隔天1次,共3次。末次染毒24 h后,腹主动脉取血,分析测定血清中白细胞介素1-β(IL-1β)、白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、超敏C反应蛋白(HS-CRP);谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、总超氧化物歧化酶(T-SOD)、血清一氧化氮(NO)、内皮素1(ET-1)和心肌缝隙连接蛋白(Cx43)的含量。结果溶剂对照组IL-1β、IL-6、TNF-α、HS-CRP、GSH、GSH-Px、MDA、T-SOD、NO、ET-1及Cx43分别为(68.73±6.21)μg/L、(15.86±0.45)μg/L、(41.12±7.66)μg/L、(1.29±0.26)μg/L、(15.30±2.52)μmol/L、(492.29±28.28)、(10.19±0.74)μmol/L、(272.98±8.59)U/m L,(3.22±0.22)μmol/L、(0.28±0.021)μg/L、(0.42±0.04)μg/L。PM_(2.5)染毒组IL-1β[(1 155.98±100.28)μg/L]、IL-6[(24.94±2.06)μg/L]、TNF-α[(821.45±14.26)μg/L]、HSCRP[(3.10±0.28)μg/L]、MDA[(15.88±1.41)μmol/L]和ET-1[(0.38±0.03)μg/L]的释放量升高,GSH[(4.62±0.37)μmol/L]、GSH-Px[(289.28±30.65)]、NO[(0.97±0.074)μmol/L]、Cx43[(0.26±0.10)μg/L和TSOD[(239.26±4.97)U/m L]含量降低,差异有统计学意义(P<0.05);与PM_(2.5)染毒组相比,VE给药组的各项指标均有一定缓解作用,差异均有统计学意义(P<0.05),且存在一定的剂量反应关系。结论急性PM_(2.5)染毒可引起大鼠心血管损伤,导致炎性因子、氧化应激指标、血管内皮功能和心肌缝隙链接蛋白的变化,而VE喂饲对PM_(2.5)引起的大鼠急性肺部损伤具有一定的保护作用。Objective To explore the intervention of vitamin E on PM_(2.5)-induced cardiovascular damage in rats. Methods Thirty-six SPF male SD rats were randomly divided into six groups including coin oil control group(solvent control group),vitamin E control group,fine particulate matter exposure group(8. 0 mg/kg·bw),VE groups with PM_(2.5) exposure(the low,middle,high experimental dose were15. 0,30. 0,60. 0 mg/kg. bw). Each experimental group was exposed to an intra-tracheal instillation for 3 times after 28 days lavage in addition to vitamin E high-dose control group and solvent group. Twenty four hours after the last exposure,the rats were sacrificed and serum was collected,then were measured the ingredients changes[Beta 1-beta interleukins(IL-1β),interleukin 6(IL-6),tumor necrosis factor alpha(TNF-alpha),high sensitive C-reaction protein(HS-CRP),Glutathione(GSH),Glutathione peroxidase(GSH-Px),Malondialdehyde(MDA),Superoxide dismutase(T-SOD),NO,Endothelin-1(ET-1) and Connexin 43(Cx43) ]. Results Compared with solvent group[(68. 73 ± 6. 21) μg/L,(15. 86 ± 0. 45)μg/L,(41. 12 ± 7. 66) μg/L,(1. 29 ± 0. 26) μg/L,(15. 30 ± 2. 52) μmol/L,(492. 29 ± 28. 28),(10. 19 ± 0. 74) μmol/L,(272. 98 ± 8. 59) U/m L,(3. 22 ± 0. 22) μmol/L,(0. 28 ± 0. 021) μg/L and(0. 42 ± 0. 04) μg/L respectively],the content of IL-1β(1 155. 98 ± 100. 28 μg/L),IL-6(24. 94 ± 2. 06μg/L),TNF-α(821. 45 ± 14. 26 μg/L),HS-CRP(3. 10 ± 0. 28 μg/L),MDA(15. 88 ± 1. 41 μmol/L) and ET-1(0. 38 ± 0. 03 μg/L) increased and the content of GSH(4. 62 ± 0. 37 μmol/L),GSH-Px(289. 28 ±30. 65) 、NO(0. 97 ± 0. 074 μmol/L),Cx43(0. 26 ± 0. 10 μg/L) and T-SOD(239. 26 ± 4. 97 U/m L)decreased in PM_(2.5) infected-group and the difference was significant between PM_(2.5) infected-group and solvent group(P < 0. 05). Compared with PM_(2.5) infected control group,the difference of various index of VE groups had statistical significance(P < 0. 05),and there was a certain dose-response relationship.Conclusion PM_(2.5) tracheal drip infections c

关 键 词：维生素E 大气细颗粒物 气管滴注 血清 心血管损伤 

分 类 号：R994.6[医药卫生—毒理学]