侯氏黑散对脑缺血大鼠神经导向因子及Rho GTP酶的影响  

Influence of Houshiheisan on the expression of axon guidance factor and Rho GTPases

作　　者：陆跃[1] 刘建红 赵晖[1] 常佳慧[1] 相阳阳 张秋霞[1] 

机构地区：[1]首都医科大学中医药学院,北京100069 [2]北京火箭军医院,北京100840

出　　处：《首都医科大学学报》2017年第6期891-896,共6页Journal of Capital Medical University

基　　金：国家自然科学基金(81373526)~~

摘　　要：目的探讨侯氏黑散对脑缺血大鼠神经导向因子Netrin-1/DCC和分子开关Rac1/Cdc42/RhoA表达的影响。方法采取线栓法制备永久性大脑中动脉栓塞(permanent middle cerebral artery occlusion,p MACO)模型。大鼠采用数字表法随机分为假手术组、模型组、侯氏黑散小剂量组、侯氏黑散中剂量组、金纳多组。运用Western blotting法和RT-PCR法检测术后7d大鼠缺血侧皮质Netrin-1、DCC、Rac1、Cdc42、RhoA蛋白和基因的表达。结果与模型组相比,侯氏黑散中剂量组、金纳多组大鼠Netrin-1蛋白的表达明显升高(P<0.01),侯氏黑散中剂量组大鼠Netrin-1基因的表达升高(P<0.05);侯氏黑散小剂量组、侯氏黑散中剂量组、金纳多组大鼠DCC、Rac1、Cdc42蛋白和基因的表达明显升高(P<0.05);侯氏黑散小剂量组、侯氏黑散中剂量组、金纳多组大鼠RhoA蛋白和基因的表达则明显下降(P<0.01)。结论侯氏黑散通过对脑缺血大鼠神经导向因子Netrin-1/DCC和分子开关Rac1/Cdc42/RhoA的调节促进脑缺血后神经功能的修复。Objective To investigate the influence of Houshiheisan on the expression of axon guidance factor Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA in cerebral ischemia injury rats.Methods The permanent middle cerebral artery occlusion（pMCAO） model was made by the intraluminal suture method.Rats were randomly divided into sham group,model group,low dose of Houshiheisan group,middle dose of Houshiheisan group and Ginaton group.Western blotting and RT-PCR were employed to detect the expression of Netrin-1,DCC,Rac1,Cdc42 and RhoA in ischemic cerebral cortex of rats 7 days after operation.Results Compared with the model group,in the middle dose of Houshiheisan group and Ginaton groups,the expression of Netrin-1 protein was elevated significantly（P0.01）,middle dose of Houshiheisan group,the expression of Netrin-1 in mRNA was significantly increased（P0.05）;in the low dose of Houshiheisan,middle dose of Houshiheisan and Ginaton groups,the expression of DCC,Rac1 and Cdc42 protein and mRNA（P0.05,P0.01） was significantly increased;but the expression of RhoA protein and mRNA was decreased significantly（P0.01）.Conclusion The findings demonstrated that Houshiheisan could accelerate the recovery of neurological impairment after celebral ischemia by regulating the expression of axon guidance factors like Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA.

关 键 词：脑缺血 侯氏黑散 NETRIN-1 DCC RAC1 CDC42 RhoA 

分 类 号：R289.5[医药卫生—方剂学]