高脂联合小剂量链脲佐菌素建立实验性2型糖尿病大鼠模型  

Study of streptozotocin and high-fat diet inducing type 2 diabetes in a rat model

作　　者：吴瑛[1] 张勇[2] 姚合斌[3] 

机构地区：[1]海军总医院营养科,北京100048 [2]中国人民解放军32150部队医院,河南新乡453000 [3]海军总医院内分泌科,北京100048

出　　处：《转化医学杂志》2017年第6期355-357,共3页Translational Medicine Journal

基　　金：解放军总后勤部保健专项课题(CWS14BJ38)

摘　　要：目的探讨建立具有胰岛素抵抗、近似人2型糖尿病特征的SD大鼠模型。方法将36只SD大鼠随机分为A组(对照组即普通饲料组)6只、B组(高脂饲料组)6只、C组[普通饲料+小剂量链脲佐菌素(streptozotocin,STZ)组]12只、D组(高脂饲料+小剂量STZ组)12只共4组。C组、D组大鼠分别在普通饲料和高脂饲料喂养4周后使用小剂量STZ(30 mg/kg)腹腔注射5 d。随机血糖>16.7 mmol/L且持续2周为建模成功。结果 C组、D组能建立2型糖尿病SD大鼠模型,成模率分别为66.7%(8/12)、91.7%(11/12)。结论高脂饲料+小剂量STZ发病过程接近自然,成模率更高,模型稳定,是值得推广的2型糖尿病动物模型。Objective To set up a SD rat model similar to human type 2 diabetes mellitus with insulin resistance. Methods Thirty-six SD rats were randomized into four groups,normal group（ group A,n = 6）,high-fat diet group（ group B,n = 6）,low-does streptozotocin group[group C,streptozotocin（ STZ）,30 mg/kg,n = 12],and high-fat diet and low-does STZ（ 30 mg/kg） group（ group D,n = 12）. Group C and D were induced by an injection of low-does STZ（ 30 mg/kg） after 4 weeks of normal chow feeding and high-fat chow feeding respectively. Rats with random plasma glucose levels 16. 7 mmol/L for 2 weeks were modeling success. Results Both normal chow feeding associated with low-does STZ（ 30 mg/kg） and high-fat chow feeding associated with low-does STZ（ 30 mg/kg） can establish type 2 diabetes animal model. The diabetes morbidity was 66. 7%（ 8/12） in group C,and 91. 7%（ 11/12） in group D. Conclusion Group D has moderately high blood sugar,insulin resistance,the success rate is high,and can be used as a good model of type 2 diabetes mellitus in relevant research.

关 键 词：2型糖尿病 血糖 高脂 链脲佐菌素 模型 大鼠 

分 类 号：R587.1[医药卫生—内分泌]