四川省嗜肺军团菌基因分型研究  

Research for gene typing of Legionella pneumophila in Sichuan province

作　　者：李红霞 姚卫 廖虹瑜[2] 罗隆泽[2] 曾林子[2] 

机构地区：[1]四川省成都市第一人民医院检验科,610041 [2]四川省疾病预防控制中心,成都610041

出　　处：《检验医学与临床》2017年第23期3458-3460,共3页Laboratory Medicine and Clinic

基　　金：四川省医学会青年创新课题(Q15081)

摘　　要：目的探讨四川省嗜肺军团菌基因特征。方法采用基因序列分型(SBT)对四川省1989-2016年分离的嗜肺军团菌共79株进行了基因型的研究。根据文献报道的军团菌7个管家基因,对79株嗜肺军团菌进行PCR扩增,SBT结果测序后上传至欧洲军团菌感染组(EWGLI)数据库得到ST分型。结果79株嗜肺军团菌分为33个ST型,其中有11个为新ST型(ST2355~ST2365)。33个ST型分为5个克隆群(CCs)和5个singleton。结论 SBT方法能对嗜肺军团菌进行基因分型研究。四川省嗜肺军团菌具备较高的遗传多样性和地区特异性,有感染人的风险,应加强对公共卫生环境中军团菌的监测工作。Objective To investigate the genetic characteristics of L.pneumophilain Sichuan province.Methods The sequencebased typing（SBT）were used to describe the genetic polymorphism of 79 strains which were isolated from 1989-2016 in Sichuan province.According to the reference,PCR was used to detect the 7 housekeeping genes,the results were sequenced and compared with the database of EWGLI.Results A total 33 sequence types（STs）were obtained with 5 main clonal complex（CCs）and 5 singleton,including 11 novel STs（ST2355-ST2365）.Conclusion SBT could be applied to study the gene typing of L.pneumophila and L.pneumophilain Sichuan show the high genetic polymorphism and the regional specificity.The results also suggest that the isolates are a potential threat to the public health,effective control and prevention strategies are urgently needed.

关 键 词：嗜肺军团菌 基因序列分型 分子检测 

分 类 号：R517.9[医药卫生—内科学]