一氧化氮参与调控镧诱导的拟南芥主根生长的生理与分子机理  

Physiological and molecular mechanisms of nitric oxide in Lanthanum-modulated primary root growth in Arabidopsis

作　　者：孙亮亮[1,2] 刘阳阳[2] 张苹[2] 王如玲[2] 马金虎[1] 徐进[2] 

机构地区：[1]山西农业大学农学院,山西太谷030801 [2]中国科学院西双版纳热带植物园,云南勐腊666303

出　　处：《山西农业大学学报（自然科学版）》2017年第12期849-853,共5页Journal of Shanxi Agricultural University(Natural Science Edition)

基　　金：国家自然科学基金(31272239);国家重点研发计划(2016YFC0501901);云南省中青年学术带头人后备人才项目(2014HB043)

摘　　要：[目的]镧(La)是一种稀土元素,La诱导了根尖ROS的累积,从而抑制了主根(PR)生长。本研究旨在探明NO是否也参与调控了La诱导的根系结构重排(RSA)。[方法]采用植物生理学和遗传学等研究方法,分析了La诱导的根尖NO累积及其生理学影响。[结果]研究表明,抑制NO的累积显著缓解了La对根尖分生区细胞分裂势的抑制,因而缓解了La诱导的PR生长抑制;进一步的遗传学分析中,我们使用NO缺陷突变体noa1证实了NO参与调控了La诱导的PR生长。抑制根尖NO的累积并不影响ROS的水平;同样抑制ROS的累积也不影响NO的水平。[结论]结果表明,La诱导的根尖NO和ROS的累积是通过相互独立的途径参与调节PR生长,因而导致了RSA。[Objective]Lanthanum（La）is one of rare earth elements that La represses primary root（PR）elongation by inducing ROS accumulation in root tips.The aim of the present study was to elucidate whether and how NO was also involved in La-mediated root system architecture remodeling（RSA）remain unclear.[Methods]For this purpose,we analyzed La-induced NO accumulation and its roles in modulating PR growth by integrating physiological studies and genetic analyses.[Results]Ourresult indicated that inhibition of NO accumulation alleviated the inhibitory effects of La on PR elongation.Genetics analysis using NO-defective mutant noa1 supported theresult.Inhibition of NO production did not affect the ROS levels,and inhibition of ROS production did not affect the NO levels.[Conclusion]The result indicated that NO and ROS signaling pathways function to modulate RSA through mutually independent pathways.

关 键 词：镧 一氧化氮 活性氧 主根生长 拟南芥 

分 类 号：S143.72[农业科学—肥料学]