PEG模拟水分胁迫下白菜型冬油菜芽期根系特征及抗旱性研究  

Research on root morphology characteristics and drought resistance of winter rapeseed ( Brassica campestris L. ) at bud stage under PEG simulated drought

作　　者：米超[1] 赵艳宁[1] 刘自刚[1] 孙万仓[1] 刘海卿[1] 方彦[1] 李学才[1] 武军燕 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,甘肃省油菜科技研究工程中心,甘肃兰州730070

出　　处：《干旱地区农业研究》2017年第5期229-235,共7页Agricultural Research in the Arid Areas

基　　金：国家现代农业产业技术体系(CARS-13);国家自然基金(31460356);国家自然基金(31660404);国家“973”计划(2015CB150206);油菜杂种优势利用技术与强优势杂交种创制(2016YFD0101300)

摘　　要：本试验采用砂培法,以18%(w/V)聚乙二醇(PEG6000)模拟水分胁迫研究18份白菜型冬油菜芽期根系特征,并结合盆栽试验研究根系特征与抗旱性关系。结果显示,水分胁迫对白菜型冬油菜侧根数、根重、根冠比有显著的抑制作用,对根长影响相对较小。相关性分析结果显示:相对侧根数、相对根长与相对活力指数之间呈显著正相关,可以作为白菜型冬油菜抗旱评价的辅助指标。不同来源的材料在根系侧根数、根重、根冠比方面差异显著,可作为区别不同来源材料抗旱性强弱的指标。对材料抗旱性进行聚类分析,在距离10时18份材料可以分五个类群,其中A类抗旱性最强,平均相对侧根数72.90%、平均相对根长100.37%、平均相对根重79.60%、平均相对根冠比119.93、平均相对活力指数0.64。根据相对活力指数及聚类分析结果,筛选出抗旱性较强的3份白菜型冬油菜:宁油2号、陇油7号和平油1号。对比分析盆栽试验,幼苗期总生物量干重与根冠比、根系干重、地上部干重、主根长均呈极显著正相关;芽期PEG胁迫下根系特征分析结果显示:早期生长活力较好的材料在盆栽试验有较高的生长势。早期采用模拟干旱的方法可以筛选抗旱性较强的材料。This study use（d） sand culture, with 18% （w/V） polyethylene glycol （PEG6000） to simulate water stress on root morphology characteristic of 18 germinating winter rapeseed, combined with pot experiment studied the root characteristics and drought resistance. The results showed that water stress had a significant inhibitory effect on root num-ber, root weight and root/shoot ratio of winter rapeseed, and had little effect on root length. The correlation analysis demonstrated that there was a significant positive correlation between the relative root number, the relative root length and the relative vigor index, and the results could be used as an assistant index for drought resistance evaluation of winter rapeseed. Drought resistance of materials cluster analysis showed that 18 materials can be divided into five groups at a distance of 10, where are the strongest drought resistance class A, the average relative lateral root number, average rela-tive root length, average relative root weight, the average relative shoot ratio, and the average relative vigor index is 72.90%, 100.37%, 79.60%, 119.93 and 0.64. Depending on the relative vigor index and cluster analysis, the 3 winter rapeseed （which are Ningyou 2, Longyou 7 and Pingyou 1） were identified as drought resistance. The results ofthe pot experiment showed that there was a significant positive correlation between the dry weight of total biomass and root/shoot ratio,root dry weight, dry weight and root length of shoots at bud stage under PEG simulated drought resis-tance, and the higher the early growth vigor, the stronger the growth potential. Therefore, the PEG simulated drought method can be uesed to f i lter the drought-resistant materials.

关 键 词：白菜型冬油菜 PEG 抗旱性 根系特征 

分 类 号：S332.1[农业科学—作物遗传育种]