外源硫化氢对加工番茄种子耐盐性及抗氧化酶的影响  

Effects of exogenous hydrogen sulfide on salt tolerance and antioxidant enzymes of processing tomato seeds

作　　者：郑州元 林海荣[1] 崔辉梅[1,2] 

机构地区：[1]石河子大学农学院,新疆石河子832003 [2]特色果蔬栽培生理与种质资源利用兵团重点实验室,新疆石河子832003

出　　处：《干旱地区农业研究》2017年第5期236-241,262,共7页Agricultural Research in the Arid Areas

基　　金：留学回国人员科技活动项目(2010LX005)

摘　　要：为明确硫化氢对盐胁迫下加工番茄种子萌发的缓解作用,用Na Cl半致死浓度为85 mmol·L^(-1)的KT-63(耐盐性弱)和半致死浓度为120 mmol·L^(-1)的KT-18(耐盐性强)为材料,用各自的半致死浓度与不同浓度的NaHS(0.2,0.4,0.6,1.0 mmol·L^(-1))分别配成混合液处理种子。结果表明,随着NaHS浓度的增加,种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性呈先上升后下降的趋势,其中与盐胁迫单独处理相比,用0.4mmol·L^(-1)NaHS处理,种子的发芽率、发芽势、发芽指数、活力指数、SOD、POD、CAT活性在KT-63中分别提升70%、250%、120%、130%、6.7%、150%和180%,在KT-18中分别提升50%、250%、60%、80%、4.3%、160%和200%,而MDA的含量显著降低,并且对KT-63的缓解效应强于KT-18。综上所述,H2S(0.4 mmol·L^(-1))有效缓解了加工番茄萌发过程中盐胁迫的抑制作用,促进了种子的萌发。The effects of exogenous hydrogen sulfide on the seed germination of processing tomato under salt stress were investigated. Seeds of two processing tomato cultivars KT - 63（salt resistance is weak） and KT - 18（stronger salt to l-erance） were treatment with semi-lethal concentration of NaCl（85 mmol·L ^-1, 120 mmol·L -1） and NaHS（0.2,0.4,0.6, 1.0 mmol·L _ 1） mixture, respectively. The results showed that： With increasing NaHS level, the germination rate, germi-nation energy, germination index, vigor index, SOD, POD and CAT activities first increased and then decreased. Com-pared with salt stress, treatments with 0.4 mmol＇L -1 NaHS significantly increased seed germination rate, germination in-dex, vigor index and the activities of SOD, POD and CAT in KT - 63 （70% , 250% , 120% , 130% ,6.1 % , 150% and 180%, respectively） and KT - 18（50% ,250% ,60% ,80% ,4.3% , 160% and 200%, respectively）, and the content of MDA was decreased. The mitigation effect of hydrogen sulfide on KT - 63 is stronger than KT - 18. Results provide evi-dence that 0.4 mmol＇L -1 of H2S may alleviate damage to seeds caused by salt stress, expedition of seed germination.

关 键 词：硫化氢 盐胁迫 加工番茄 种子萌发 抗氧化酶 

分 类 号：S641.2[农业科学—蔬菜学] S603.4[农业科学—园艺学]