山羊体细胞重编程过程中慢病毒对细胞的毒性作用  

Cytotoxicity of Lentivirus to Goat during Somatic Reprogramming

作　　者：宋辉[1,2] 李卉[1,2] 王锋 

机构地区：[1]宁夏医科大学生育力保持省部共建教育部重点实验室,宁夏银川750004 [2]南京农业大学江苏省肉羊产业工程技术研究中心,江苏南京210095

出　　处：《安徽农业科学》2017年第35期82-84,共3页Journal of Anhui Agricultural Sciences

基　　金：教育部"春晖计划"(Z2016044);高教一流学科师资队伍建设(30181601);宁夏高等学校科学技术研究项目(NGY2016090)

摘　　要：[目的]检测慢病毒对普通山羊、克隆奶山羊和转基因克隆奶山羊的作用.[方法]采用慢病毒作为载体感染普通山羊、克隆奶山羊(♂12001)和转基因克隆奶山羊(♀12003)体细胞,通过细胞生长曲线,检测慢病毒对3种山羊细胞的毒性作用.[结果]感染后的3种体细胞在感染前3 d,细胞大量凋亡,4~7 d筛选出的细胞大量增殖.未经慢病毒感染的3种细胞接种2 d后数量开始明显增加,第25天进入对数生长期,第57天进入平台期,呈现典型的"潜伏期-对数生长期-停滞期"生长模式.[结论]慢病毒对细胞有毒性作用,会引起细胞大量凋亡.[ Objective]The aim is to detect the role of lentivirus in normal goats, cloned dairy goats and transgenic cloned dairy goats. [ Meth-ods] Using lentiviral vector infection as a common goat, cloned goats （male 12001） and transgenic cloned goats （female 12003） somatic cells by cell growth curve, toxic effect of lentivirus on three kinds of goat cell was detected . [ Result ] After infection, the three somatic cells were infected with 3 days, and the apoptosis of cells was large. The cells screened from 4 d to 7d proliferated greatly. Without the lentivirus infected three cells significantly increased after inoculation with 2 d, cells entered the logarithmic growth phase from 2rd day to 5th day, then entered the platform phase from 5 th day to 7 th day, it was a typical a latency-logarithmic growth period - the period of stagnation” growth mode.[Conclusion] Lentivirus plays a toxic role in cells and can cause apoptosis in a large number of cells.

关 键 词：慢病毒 细胞生长曲线 凋亡 

分 类 号：S858.27[农业科学—临床兽医学]