潜伏性感染者和结核病患者T细胞亚群产生干扰素-γ的特点及意义  

The characteristics and significance of T cell subpopulations producing IFN-γ in latent infected people and TB patients

作　　者：盛玲玲[1] 金辉[1] 彭宁[1] 盛显仓 杨再兴[1] 李柏青[2] 

机构地区：[1]台州市第一人民医院,浙江台州318020 [2]安徽省蚌埠医学院免疫学教研室,安徽蚌埠233030

出　　处：《中国卫生检验杂志》2017年第22期3244-3246,3249,共4页Chinese Journal of Health Laboratory Technology

基　　金：卫生部重大传染病科技重大专项课题(2012ZX1000-3006-004)

摘　　要：目的观察磷酸化抗原(HDMAPP)、结核杆菌耐热抗原(Mtb-HAg)和结核杆菌低分子耐热抗原Mtb-HAg(10 k D)刺激结核分枝杆菌潜伏性感染者(LTBI)和TB患者外周血T细胞亚群产生IFN-γ的反应能力方面的特点及差异。方法采集LTBI和TB患者外周血,分别用HDMAPP、Mtb-HAg和Mtb-HAg(10 k D)刺激培养14 h,再加入莫能霉素继续培养6 h,收集细胞,加入荧光标记单抗进行表面分子和胞内染色,然后在流式细胞仪上检测各实验组中CD3+αβ细胞和CD3+γδ细胞胞内IFN-γ的表达。结果各抗原刺激组T细胞亚群均能产生IFN-γ,各抗原刺激组LTBI和TB患者的外周血γδT细胞中产生IFN-γ的细胞均显著高于αβT细胞;LTBIγδT细胞中产生IFN-γ细胞均比TB患者高,上述差异均有统计学意义(P<0.05)。结论γδT细胞的IFN-γ在LTBI和TB患者的不同,有望为区分LTBI和TB患者之间的差异提供新的指标。Objective To observe the characteristics and differences of reaction ability in T cell subpopulations producing IFN-γ in latent infected people（ LTBI） and TB patients,when HDMAPP,Mycobacterium tuberculosis heat-resistant antigen（ Mtb-HAg） and low molecular peptide antigen of Mycobacterium tuberculosis（ Mtb） were used to stimulate the peripheral blood T cells.Methods The peripheral blood cells from LTBI and TB patients were collected for the stimulation and culturing with HDMAPP and Mtb-HAg and Mtb-HAg（ 10 k D） for 14 hours,and then adding Monensin to cultivate for another 6 hours. Cells were collected and stained with different fluorochrome-conjugated m Abs for staining of surface molecule and intracellular cytokines. Then the expressions of IFN-γ of CD3~＋αβ cells and CD3~＋γδ cells among the experimental groups were detected by flow cytometry.Results The T cell subpopulations among antigen experimental groups can all produce IFN-γ. The γδ T cells producing IFN-γ were more than αβ cells among the LTBI antigen experimental groups（ P〈 0. 05）. The γδ T cells producing IFN-γ in LTBI patients were more than TB patients,with all the differences above statistically significant（ P〈 0. 05）. Conclusion The difference of γδ T cells producing IFN-γ between in LTBI and TB patients is expected to provide new indicators for distinguishing latent infected people and TB patients.

关 键 词：结核分枝杆菌 潜伏性感染者 干扰素 ΓΔT细胞 ΑΒT细胞 

分 类 号：R392[医药卫生—免疫学]