海蛇药酒对Ⅱ型胶原诱导关节炎大鼠外周血中CD4^+/CD8^+及IgA、IgM、IgG的影响  

Influence of Sea Snake Wine on CD4^+/CD8^+ and IgA,IgM,IgG in peripheral blood of type Ⅱ collageninduced rheumatoid arthritis rats

作　　者：王宁宁[1] 戴莹[1] 王威[1] 张硕峰[1] 何忠平[2] 梁晓琪 孔李婷 贾晓玉[1] 翟华强[1,3] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北海国发海洋生物产业股份有限公司制药厂,北海536000 [3]北京师范大学资源学院资源生态与中药资源研究所,北京100875

出　　处：《北京中医药》2017年第10期904-908,F0003,共6页Beijing Journal of Traditional Chinese Medicine

基　　金：中国博士后科学基金资助项目(2012M510346);国家自然科学基金项目(81373887)

摘　　要：目的研究海蛇药酒对Ⅱ型胶原诱导关节炎(CIA)大鼠外周血中CD4^+/CD8^+及IgA、IgM、IgG的影响,探讨其对类风湿关节炎(RA)的治疗作用。方法设立正常组、模型组、阳性对照组及海蛇药酒组,建立Ⅱ型CIA大鼠模型。应用流式细胞仪检测大鼠外周血中CD4^+、CD8^+值,ELISA法检测大鼠外周血中IgA、IgM、IgG水平。结果与正常组比较,模型组CD4^+/CD8^+值、IgM、IgA、IgG水平均升高(P<0.05);与模型组比较,阳性对照组与海蛇药酒组CD4^+/CD8^+值、IgM、IgA、IgG水平均降低(P<0.05);与阳性对照组比较,海蛇药酒组CD8^+值降低,IgM及IgG水平值升高,差异有统计学意义(P<0.05),其他指标比较差异无统计学意义(P>0.05)。结论海蛇药酒可能通过纠正CD4^+/CD8^+的失衡而调节RA免疫功能紊乱,降低IgM、IgA、IgG水平,抑制免疫球蛋白的分泌及炎症反应,达到治疗RA的作用。Objective To study the effects of Sea Snake Wine on CIM ＋/CD8 ＋ , IgA, IgM, IgG in peripheral blood of type II collagen-induced arthritis （CIA） rats, and the therapeutic effects of it on RA. Methods Normal control group, model group, positive control group, and Sea Snake Wine group were randomly divided, and type CIA rat model was established. The flow cytometry was used to detect the levels of CD4 ＋ and CD8 ＋ , and the contents of IgA, IgM and IgG were measured by ELISA. Results Compared with the normal group, the level of CD4 ＋/CD8 ＋ and IgM, IgA, IgG of the model group were increased （ P 〈 0. 05 ）. Compared with the model group, the levels of CIM＋/CD8＋ and IgM, lgA and IgG of the positive control group and the Sea Snake Wine group were decreased （ P 〈 0. 05 ） ; compared with the positive control group, the levels of CD8 ＋ and IgM and IgG of Sea Snake Wine group was increased, the difference was statistically significant（ P 〈 0. 05 ）. There were no significant differences between the other values（ P 〉 0.05 ）. Conclusion The Sea Snake Wine could correct CD4 ＋/CD8 ＋ imbalance to regulate the disorder of immune system in rheumatoid arthritis （RA） , reduce the level of IgA, IgM, IgG, and inhibit the secretion of immunoglobulin and inflammatory reaction so as to treat arthritis.

关 键 词：海蛇药酒 类风湿关节炎 Ⅱ型胶原诱导关节炎 CD4+ CD8+ 免疫球蛋白 大鼠 

分 类 号：R285.5[医药卫生—中药学]