人乳头瘤病毒16型E7抗原细胞毒性T细胞预测表位的分子模拟研究  

Study of molecular mimicry of predicted cytotoxic T lymphocyte epitopes derived from HPV16 E7 antigen

作　　者：徐云升[1] 郝飞[1] 耿淼[2] 叶庆佾[1] 钟白玉[1] 

机构地区：[1]第三军医大学西南医院皮肤科,重庆400038 [2]第三军医大学全军免疫研究所,重庆400038

出　　处：《临床皮肤科杂志》2002年第9期545-547,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助课题(编号:30070698)

摘　　要：对人乳头瘤病毒(HPV)16型E7抗原的预测表位从理论上分析其与HLA-A2分子结合的情况,推测成为HLA-A2限制性表位的可能性。通过计算机分子模拟,确立各表位及其与HLA-A2分子结合形成复合物的模拟结构。结果发现,各表位的三维结构符合HLA-A2限制性细胞毒性T细胞(CTL)表位的结构要求,能较好地与HLA-A2分子结合。根据分子模拟提供的理论支持,各预测表位均符合要求,提示可在后续实验中进行表位合成、筛选、鉴定和多肽疫苗的研制。To analyze the binding of cytotoxic T lymphocyte epitope candidates derived from HPV16E7to the HLA-A2molecule and the possibility of them being HLA-A2-restricted CTL epitopes,the molecular mimicry of CTL epitope candidates bound to the HLA-A2molecules and of the HLA-A2-peptide complex was established by a computer molecular modeling.The results showed that the three-dimensional configurations of HLA-A2binding peptides were compatible with those of HLA-A2-restricted CTL epitopes and they could bind to HLA-A2molecules quite well.According to mole cular modeling,it is suggested that the predicted peptides may be the CTL epitopes restricted by HLA-A2molecules,and can be useful in the study of syn-thesis,screening,identification and preparing peptide vaccine.

关 键 词：人乳头瘤病毒 E7抗原 细胞毒性T细胞表位 分子模拟 

分 类 号：R392.11[医药卫生—免疫学]