支气管哮喘患者外周血单个核细胞TIPE2、TF的表达  

Expressions of TIPE2 and TF in peripheral blood mononuclear cells of patients with bronchial asthma

作　　者：时光[1,2,3] 赵俊伟 明亮[1,2,3] 

机构地区：[1]郑州大学第一附属医院检验科 [2]河南省检验医学重点实验室 [3]郑州大学医学检验系,郑州450052

出　　处：《郑州大学学报（医学版）》2017年第6期685-689,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目81501715;河南省科技攻关项目162102310142;152102410067

摘　　要：目的:检测支气管哮喘患者外周血单个核细胞(PBMCs)肿瘤坏死因子-α诱导蛋白-8样因子-2(TIPE2)、组织因子(TF)表达水平及二者的相关性,并探讨二者与哮喘控制水平的相关性。方法:108例哮喘患者根据哮喘控制水平分为未控制组53例,部分控制组55例,另选择同时期健康体检者51人(健康对照组)。Western blot法检测3组患者PBMCs中TIPE2、TF的表达水平,Spearman秩相关分析二者的相关性,受试者工作曲线评估TIPE2和TF对支气管哮喘的诊断价值。结果:与部分控制组和健康对照组相比,未控制组PBMCs中TIPE2表达水平降低(P<0.01);与健康对照组相比,部分控制组PBMCs中TIPE2表达水平降低(P<0.01)。与健康对照组相比,部分控制组和未控制组PBMCs中TF表达水平升高(P<0.01),而二组间差异无统计学意义(P>0.05)。Aim： To detect the expression levels of tumor necrosis factor-α induced protein 8 like 2（ TIPE2） and tissue factor（ TF） in peripheral blood mononuclear cells（ PBMCs） of patients with bronchial asthma,and to investigate the relationship between TF or TIPE2 with asthma control level. Methods： One hundred and eight cases of asthma were allocated into two groups： the partial control group（ 55 cases） and the uncontrolled group（ 53 cases）,and 51 healthy persons were selected as healthy control group. The expression levels of TIPE2 and TF in PBMCs were detected by Western blot. Correlation between TF and TIPE2 was analyzed by Spearman correlation. Evaluation of the diagnostic value of TIPE2 and TF in bronchial asthma was operated by receiver operating curve. Results： Compared with healthy control and partial control groups,the expression level of TIPE2 in PBMCs of uncontrolled group was significantly lower（ P〈0. 01）,which was lower in partial control group than that in healthy control group（ P〈0. 01）. Compared with healthy control group,the expression level of TF in partial control group and uncontrolled group was significantly higher（ P〈0. 01）,and there existed no significant difference between partial control group and uncontrolled group（ P〈0. 05）. The expression of TIPE2 was negatively correlated with TF in PBMCs of asthma patients（ r =-0. 414,P〈0. 001）. When,determining TIPE2 less than 0. 364,TF value greater than 0. 354 as the distinction between asthma patients and healthy subjects,the sensitivity and specificity of diagnosis of asthma were 68. 4%,95. 5% for TIPE2,and 39. 5%,100. 0% for TF,respectively. Using TIPE2 less than 0. 115 as dividing line between asthma partial control group and asthma uncontrolled group,the sensitivity and specificity were 76. 7% and 100. 0%,respectively. Conclusion： The expression of TIPE2 in PBMCs of patients with asthma is lower,while that of TF is higher. The TIPE2 expression in PBMCs of asthma patients is negatively corre

关 键 词：支气管哮喘 肿瘤坏死因子-α诱导蛋白-8样因子-2 组织因子 

分 类 号：R562.2[医药卫生—呼吸系统]