木鳖子中丝石竹皂苷元-3-O-β-D-葡萄糖醛酸甲酯测定  

Determination of gypsogenin-3-O-β-D-glucuronide methyl ester in momordicae semen

作　　者：郑蕾 申旭霁 张寒 张彦 汪兴军 

机构地区：[1]西安医学院药学院药物研究所,陕西西安710021

出　　处：《化工科技》2017年第6期58-61,共4页Science & Technology in Chemical Industry

基　　金：陕西省自然科学基础研究计划项目(2017JQ8042);西安医学院科研项目(2016GJFY06;2016GJFY07;2016DXS1-09;2016DXS2-03)

摘　　要：建立测定木鳖子中丝石竹皂苷元-3-O-β-D-葡萄糖醛酸甲酯高效液相色谱法,并对木鳖子不同部位进行测定。色谱条件为Agilent HC-C18(4.6mm×250mm,5μm)色谱柱,甲醇-φ(甲酸)=0.2%水溶液(体积比为78∶22)为流动相,流速1.0mL/min,检测波长210nm,室温测定。结果显示丝石竹皂苷元-3-O-β-D-葡萄糖醛酸甲酯质量浓度在6.44×10-2~1.03mg/mL呈良好线性关系(r=0.999 7)。该方法操作简便、重复性好、准确性好,可用于木鳖子药材炮制方法研究过程中的质量控制。The method for the determination of gypsogenin-3-O-β-D-glucuronide methyl ester in momordicae semen by HPLC was established.The assay was conducted on Agilent HC-C18(4.6 mm×250 mm,5μm),with methanol-water(containing 0.2% formic acid)volume ratio of 78∶22 as mobile phase,with the flow rate of 1.0 mL/min.The detection wavelength was 210 nm at room temperature.The result showed that the linear range of gypsogenin-3-O-β-D-glucuronide methyl ester was 6.44×10-2~1.03 mg/mL(r=0.999 7).This method is convenient,repeatable and accurate,and it can be used for the quality control of momordicae semen.

关 键 词：木鳖子 丝石竹皂苷元-3-O-β-D-葡萄糖醛酸甲酯 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]