326例尖锐湿疣患者肛门及肛管组织HPV基因型检测及性别对比研究  

HPV genotype detection and the constrast research of male and female on 326 cases with anus and anal canal condyloma acuminata tissue

作　　者：张金浩[1] 耿建祥[2] 路斌 吴崑岚[1] 鞠梅 时磊 李良 李东斌[2] 赵雪[2] 

机构地区：[1]南京中医药大学第三附属医院全国肛肠医疗中心,南京医学硕士210001 [2]南京中医药大学第三附属医院病理科,南京210001 [3]周口市中医院皮肤科,周口466002 [4]中国医学科学院北京协和医学院皮肤病医院理疗科,南京210042

出　　处：《医学研究生学报》2017年第12期1246-1249,共4页Journal of Medical Postgraduates

基　　金：江苏省南京市卫计委中医专项支助项目(2009-92)

摘　　要：目的国内有关肛门及肛管尖锐湿疣(CA)组织HPV分型研究的大样本资料报道极少。文中旨在探讨以江苏省为主的区域性男女肛门及肛管CA组织中HPV感染基因型分布状况和分型的临床意义。方法收集1985年8月至2017年7月江苏省和安徽省4家医院病理组织学诊断为两性肛门及肛管乳头瘤样增生伴挖空细胞(疑似CA)的石蜡组织标本326例。从其CA组织中抽提HPV DNA,对HPV感染型别分布状况进行分析。结果 326例两性肛门及肛管CA患者中,HPV总检出率为74.85%(244/326)。其中女147例,男179例,HPV总检出率分别为73.47%、75.98%。两性肛门及肛管CA组织阳性者检出不同型别HPV出现频数合计342次,低危型HPV出现频数合计267次,高危型HPV出现频数合计75次。其中6、11、16、18型HPV感染频数占前4位。30岁以下女性CA发病率较男性显著升高(32.65%vs 16.20%,P<0.05)。结论两性肛门及肛管CA组织中,以6、11、16、18型HPV感染最为常见;30岁以下女性CA发病率较高,应引起疾病预防和临床诊治的高度重视。Objective Large sample data of HPV genotyping on anus and anal canal condyloma acuminata( CA) tissue were rarely reported in China. This study is aimed to investigate the clinical value of the distribution of different kinds of genotype of human papillomavirus within regional( mainly in Jiangsu Province) patients of both male and female with anus and anal canal condyloma acuminata( CA). Methods We collected tissue specimens from326 male and female patients with anus and anal canal condyloma acuminata( CA) from 4 hospitals in Jiangsu and Anhui provinces from August 1985 to July 2017. HPV DNA was extracted and through the combination of gene chip and polymerase chain reaction technology,23 genotypes of HPV were detected. Results Among 179 male cases,136 were found with HPV. The total HPV detection rate was 75.98%( 136/179),and of which,single genotype detection rate was55.31%( 99/179) while multiple genotypes detection rate was 20.67%( 37/179).Meanwhile,in the 147 female cases,108 cases with HPV represented the total HPV detection rate of 73.47%( 108/147),of which single genotype detection rate was 48.79%( 72/147) and multiple genotypes detection rate was 24.49%( 36/147).342 were detected for all the genotype frequency totally and the low-risk HPV genotype was appered for 267 times while 75 times for high-risk HPV in this term. Among all the genotypes,6,11,16,18 took the top 4 respectively. Conclusion Among the regional( mainly in Jiangsu Province) male and female cases with anus and anal canal( CA),HPV 6,11,16,18 were the most common types. HPV was mostly distributed in the population of male younger than 30 years old.

关 键 词：肛门及肛管 组织 尖锐湿疣 人乳头瘤病毒 基因分型 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]