多肽探针结合纳米银催化反应-吸收测定HCG  

Peptide Probe Combined Nanosilver Catalytic Reaction-Surface Plasmon Resonance Spectrophotometric Detection for Trace Human Chorionic Gonadotropin

作　　者：李重宁 潘宏程[1] 刘庆业[2] 梁爱惠[2] 蒋治良[2] 

机构地区：[1]桂林理工大学化学与生物工程学院,广西桂林541006 [2]广西师范大学环境与资源学院,广西桂林541004

出　　处：《广西师范大学学报（自然科学版）》2017年第4期91-97,共7页Journal of Guangxi Normal University:Natural Science Edition

基　　金：国家自然科学基金(21667006;21567005;21465006;21477025;21367005);广西自然科学基金(2013GXNSFFA019003)

摘　　要：在pH7.4的Na_2HPO_4-NaH_2PO_4缓冲溶液及人绒毛膜促性腺激素多肽探针(HCGP)存在下,银纳米粒子(AgNPs)发生聚集;当加入人绒毛膜促性腺激素(HCG)后,形成稳定的HCGP-HCG复合物,AgNPs能够稳定地分散在溶液中。该多肽反应液中分散的AgNPs对H_2O_2还原HAuCl_4生成金纳米粒子的反应具有较强的催化作用,其产物金纳米微粒在550nm处有一较强的表面等离子体共振(SPR)吸收峰。随着HCG浓度增大,反应液中分散的AgNPs增加,催化作用增强,550nm处的吸光度增大。HCG浓度在0.5~15μg/L与吸光度增大值ΔA_(550nm)成线性,检出限为0.2μg/L。据此,建立了简便、价廉、灵敏检测HCG的多肽探针纳米银催化光度新方法。In pH 7.4 Na2HPO4-NaH2PO4 buffer solution with the presence of human chorionic gonadotropin peptide（HCGP）probe,the AgNPs can be aggregated to bigger AgNPs clusters.However,AgNPs can be well dispersed into this solution when the human chorionic gonadotropin（HCG）is added to form stable HCGP-HCG composite.The well-dispersed AgNPs exhibits catalysis on the reaction of H2O2 reducting HAuCl4 into AuNPs which appeares a strong surface plasmon resonance（SPR）peak at 550 nm.With the increase of HCG concentration,the SPR peak increases linearly at 550 nm.The increased SPR absorption（ΔA（550 nm））is linear to HCG in the range of 0.25～50μg/L,with a regression equation of ΔA（550 nm）=0.012 4 C＋0.02,a correlation coefficient of 0.987 8,and a detection limit of 0.2 μg/L.Thus,a simple,low-cost and sensitive nanosilver-catalytic SPR spectrometry was developed for the determination of HCG.

关 键 词：多肽探针 人绒毛膜促性腺激素 AGNPS 纳米催化 SPR光度法 

分 类 号：O657.3[理学—分析化学]