牵牛E3泛素连接酶PNRma1基因的克隆及抗旱相关性分析  

Cloning and Drought Resistance Analysis of E3 Ubiquitin Ligase PNRma1 Gene in Pharbitis nil L.

作　　者：代锦绣 董轩名 倪欢欢 董延龙[2] 金晓霞[1] 

机构地区：[1]哈尔滨师范大学,哈尔滨150025 [2]黑龙江省农业科学院园艺分院,哈尔滨150069

出　　处：《中国林副特产》2017年第6期6-10,共5页Forest By-product and Speciality in China

基　　金：黑龙江省大学生创新训练计划项目(201510231017)

摘　　要：为研究牵牛E3泛素连接酶对其干旱胁迫的响应情况,利用RT-PCR技术从牵牛中克隆得到1个E3泛素蛋白连接酶基因PNRma1的cDNA全长序列,其序列长度为734bp,编码244个氨基酸。对牵牛PNRma1蛋白进行二级结构预测,结果表明该蛋白主要由α螺旋(41.88%)和无规则卷曲(37.18%)构成,并且牵牛PNRma1蛋白与野生潘那利番茄的同源性最高(相似度为94%)。在干旱胁迫处理下,PNRma1基因在牵牛根部的表达量总体上与对照相比呈现上升后下降的趋势,其在叶片中表达水平呈现后期上升趋势,试验结果表明,PNRma1基因响应牵牛的干旱胁迫反应,并且在根部(6h)早于叶片(9h)应答干旱信号。The experiment cloned an cDNA sequence of E3 ubiquitin ligase gene PNRma1 by RT-PCR method with Pharbitis nil L.used as experimental materials.The length of LeRma1 gene was 734 bp,which encoded 244 amino acids.Two stage structure of PNRma1 protein was predicted and the results showed that the protein was mainly composed of alpha helix(41.88% )and irregular coiled coil(37.18% ).Homology analysis showed that the deduced amino acid sequence exhibited high homology with Solanum pennellii(94% similarity).Under drought stress,the expression of PNRma1 gene compared with the control in the root showed a trend of increase and then decrease,and its expression level in leaves showed upward trend later,the results showed that the PNRma1 gene responded the drought stress response of morning glory and responded to drought signals at the root(6 h)earlier than the leaf(9 h)response.

关 键 词：牵牛 PNRma1 生物信息学 表达分析 

分 类 号：Q814[生物学—生物工程]