黄连属植物DNA条形码研究  

DNA Barcoding Evaluation in the Genus Coptis Salisb.

作　　者：李波 刘俊 闵道长 何小慧 李波超 朱昌兰[1] 张志勇[1] 袁庆军[2] 吴丁 

机构地区：[1]江西农业大学农学院,江西南昌330045 [2]中国中医科学院中药资源中心,北京100700 [3]景德镇学院,江西景德镇333000

出　　处：《江西农业大学学报》2017年第6期1089-1095,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31360046);国家级大学生创新创业训练计划项目(201510410006)~~

摘　　要：本研究基于黄连属(Coptis Salisb.)的大尺度取样,全面评估了叶绿体DNA序列psb A-trn H和ycf1,及核DNA序列ITS和ETS在黄连属物种鉴定中的有效性。综合分析了不同候选序列的特征,计算种内种间遗传距离,评估序列的条形码间距,及构建邻接树,发现各片段种内变异率都远小于种间变异率,4个片段都不存在明显的条形码间距,单一片段鉴定效率仅在47%~63%,而片段组合中,双片段组合ITS+ycf1具有最高的分辨率(达到79%),与3片段组合ITS+ETS+ycf1及4片段组合(ITS+ETS+ycf1+psb A-trn H)鉴定率相同,且能成功鉴定中药黄连的原植物,即三角叶黄连(C.deltoidea)、云南黄连(C.teeta)和黄连(C.chinensis)。因此,笔者建议将ITS+ycf1序列组合作为中药黄连鉴定的标准条形码。The present study made a global samling of Coptis and selected four frequently used DNA barcodes（ trn H-psb A and ycf1 from cp DNA,ITS and ETS from nr DNA）,to evaluate their species discrimination ability in the genus.Based on the comprehensive analyses of the sequence characteristics,intra/inter-specific genetic distance,DNA barcoding gap and neighbor-joining trees,the results show that the intra-specific genetic distance in each barcode was lower than inter-specific genetic distance,no obvious ＂barcoding gap＂was observed in every barcode,and species identification efficience of each barcode varied from 47% to 63%.Among the barcode combinations,the ITS＋ycf1 has the same resolution as combining three or four barcodes,and the identification efficience is up to 79%,indicating that the ITS＋ycf1 barcode combination would be valuable to be selected as the standard DNA barcode for Coptis species identification.

关 键 词：DNA条形码 分子鉴定 中药 黄连属 

分 类 号：Q943[生物学—植物学]