玉米干种子基因组DNA提取方法的改进  

Improvement of Genome DNA Extraction Method from Maize Dry Seeds

作　　者：董永军 王陆军[2,3] 郝建平 王艳梅[2,3] 李培良[2,3] 王创云 邓艳芳[3] 周琼 李志敏[3] 庞冰 张婷婷 

机构地区：[1]山西大学生命科学学院,山西太原030006 [2]山西省农业科学院作物科学研究所,山西太原030031 [3]山西省玉米工程技术研究中心,山西太原030031

出　　处：《山西农业科学》2017年第12期1903-1906,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家星火计划项目(2015GA630001);山西省工程技术研究中心项目(201605D141005);山西省农业科学院种业发展专项(2016ZYZX08);山西省重点研发计划项目(201603D221002-7);山西省重点研发计划项目(201703D221001-2);山西省科技成果转化引导专项(201604D131037)

摘　　要：为了实现玉米干种子基因组DNA快速提取,针对玉米干种子淀粉含量高的特点,对传统核酸CTAB提取方法进行了优化与改良,将提取的DNA进行分光光度计及琼脂糖凝胶电泳检测。结果显示,DNA的OD_(260)/OD280值为1.79~1.95,OD_(260)/OD230值为1.90~2.15;琼脂糖凝胶电泳图谱清晰,条带无拖尾现象,说明采用改良方法提取的DNA质量高、无降解。以改良方法提取的DNA样品作为模板进行分子标记检测试验,结果清晰稳定,进一步证明采用改良CTAB法直接从玉米干种子中提取的DNA可以满足SSR和SNP等分子试验的要求。To realize the rapid extraction of maize dry seed genomic DNA, according to the characteristics of high starch content inmaize dry seeds, this study optimized and improved the extraction method of traditional nucleic acid CTAB, and analyzed the extractedDNA by spectrophotometer and agarose gel electrophoresis. The results showed that the OD 260/ OD 280 value of DNA was between1.79-1.95, and the OD260/OD230 value was between 1.90-2.15. The gel electrophoresis pattern was clear and the band was no tailing,which illustrated that the improved DNA was high degradation. DNA samples extracted by the new method were used as templates todetect the molecular markers, the results were clear and stable. It further proved that DNA extracted from maize dry seeds by modifiedCTAB method could meet the requirements of SSR and SNP.

关 键 词：玉米 干种子 DNA CTAB 

分 类 号：S513[农业科学—作物学]