醒脑再造胶囊对大鼠脑缺血再灌注损伤的保护作用  

Protective effects of Xingnao Zaizao capsules on cerebral ischemia reperfusion injury in rats

作　　者：李向阳[1,2] 苏慧[1,2] 姚永琴[1] 吴光泽[2] 徐庆锋 吴芹 

机构地区：[1]商丘医学高等专科学校药理学教研室,河南商丘476005 [2]遵义医学院贵州省基础药理学省部共建教育部重点实验室,贵州遵义563003

出　　处：《华西药学杂志》2017年第6期586-588,共3页West China Journal of Pharmaceutical Sciences

基　　金：河南省教育厅高等学校重点科研项目(编号:15B310012)

摘　　要：目的观察醒脑再造胶囊对大鼠脑缺血再灌注损伤的保护作用。方法 ig给予SD大鼠醒脑再造胶囊7 d后,建立大鼠大脑中动脉缺血2 h再灌注24 h模型,观察各组大鼠的神经功能评分,测定脑梗死百分比和脑组织的含水量;实时荧光定量PCR检测脑组织中缺血半暗带区域核因子相关因子2(NRF2)和NAD(P)H∶醌氧化还原酶1(NQO1)mRNA的表达。结果经醒脑再造胶囊预处理可明显降低大鼠脑缺血再灌注损伤后的神经功能评分、脑梗死百分比和脑组织的含水量,并使脑组织缺血半暗带区域NRF2和NQO1 mRNA的表达显著增加。结论醒脑再造胶囊对大鼠脑缺血再灌注损伤有保护作用,其作用机制与激活脑组织中NRF2-ARE抗氧化损伤通路基因的表达有关。OBJECTIVE To observe the protective effects of Xingnao Zaizao capsules （XNZZC） on cerebral ischemia reperfusion injury in rats. METHODS After SD rats were given XNZZC for 7 days,the rat model of cerebral isehemia 2 h reperfusion 24 h was established. Effects of XNZZC on neurological scale,percentage of cerebral infarction,the water content in brain tissue and the mRNA expressions of NRF2 and NQO1 of rats were observed. RESULTS XNZZC could obviously reduce neurological scale, percentage of cerebral infarction and the water content in brain tissue,and it could increase the mRNA expressions of NRF2 and NQO1 of the rats after cerebral ischemia reperfusion injury. CONCLUSION The protective effects of XNZZC on cerebral isehemia reperfusion iujury may be associated with activation of the NRF2 - ARE antioxidant gene pathway.

关 键 词：醒脑再造胶囊 脑缺血再灌注损伤 核因子相关因子2 NAD(P)H 醌氧化还原酶1 氧化损伤 抗氧化反应元件 

分 类 号：R96[医药卫生—药理学]