晚卵泡期高孕酮对卵巢正常反应者子宫内膜胞饮突、整合素、白血病抑制因子、白介素-1影响研究  

Effects of high progesterone in late follicular phase on pinopode and the expression of integrin,LIF and IL-1 in endometrium of patients with normal ovarian responses

作　　者：李朋粉[1] 谭丽[1] 马丽影[1] 赵冬梅[1] 

机构地区：[1]郑州大学第二附属医院生殖医学中心,河南郑州450014

出　　处：《中国实用妇科与产科杂志》2017年第12期1267-1270,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：河南省科技厅/郑州市科技局项目(161202310207/153pkjgg163)

摘　　要：目的探讨晚卵泡期高孕酮(P)对卵巢正常反应者种植窗期内膜胞饮突、整合素、白血病抑制因子(LIF)及白介素-1(IL-1)表达的影响。方法选取2014年4月至2015年12月在郑州大学第二附属医院行长方案体外受精-胚胎移植(IVF-ET)助孕的29例卵巢正常反应患者,在本次取卵周期取消移植。按照h CG日孕酮水平分为:高P组(≥7.13nmol/L)18例,P未升高组(<7.13nmol/L)11例。取卵后5 d采集内膜组织,扫描电镜观察内膜胞饮突变化,Western blot法检测两组子宫内膜整合素、LIF、IL-1蛋白表达变化。结果内膜胞饮突在高P组出现皱缩,P未升高组呈发育中及完全发育状态;高P组内膜整合素(0.227±0.060 vs.0.512±0.031)、LIF(0.144±0.018 vs.0.474±0.041)、IL-1(0.220±0.009 vs.0.460±0.035)蛋白表达量均低于P未升高组(P<0.05)。结论 h CG日高孕酮改变了卵巢正常反应人群种植窗期内膜胞饮突、整合素、LIF及IL-1的表达,可能使种植窗期子宫内膜的发育提早成熟,导致胚胎-子宫内膜对话失败。Objective To explore the changes of pinopode and the expression of integrin,LIF and IL-1 in endometrium under high levels of progesterone in late follicular phase in patients with normal ovarian responses. Methods This was a prospective analysis conducted in the Reproductive Medical Center of the Second Affiliated Hospital of Zhengzhou University.Totally 29 patients with normal ovarian responses were collected,who received long agonist program treatments in IVF-ET cycles and canceled embryo transfer from April 2014 to December 2015.Patients were divided into two groups according to their serum progesterone concentration on the day of h CG administration：≥7.13 nmol/L（high P group,n=18）and 〈7.13 nmol/L（normal P group,n=11）.Endometrial biopsies were performed 5 days after oocyte retrieval. The changes of pinopodes were observed by scanning electron microscope. The expression of integrin,LIF and IL-1 was detected by Western blot method.Results The pinopodes were wrinkled in the high P group,but in the normal P group they were in a state of development and maturation.The expression of integrin（0.227±0.060 vs. 0.512±0.031）,LIF（0.144±0.018 vs. 0.474±0.041）and IL-1（0.220±0.009 vs. 0.460±0.035）in the high P group were lower than the normal group（P 〈0.05）.Conclusion High P level on the day of h CG administration may affect the expression of pinopodes and integrin,LIF and IL-1 in the peri-implantation period of these patients with normal ovarian responses.The elevated progesterone may lead to the early maturation of the endometrium during implantation phase,leading to a failure of the embryo-endometrium dialogue.

关 键 词：孕酮 体外受精-胚胎移植 子宫内膜容受性 胞饮突 整合素 白血病抑制因子 白介素-1 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]