11种植物提取物抗烟草花叶病毒活性研究  

Anti-Tobacco Mosaic Virus Activity of 11 Plant Extracts

作　　者：祁进康 邹正彪 王德艳 毕再涛 钱瑞 陈永对[2] 闫晓慧 

机构地区：[1]西南林业大学生物多样性保护与利用学院,云南省森林灾害预警与控制重点实验室,云南昆明650224 [2]云南省农业科学院生物技术与种质资源研究所,云南省农业生物技术重点实验室,云南昆明650223

出　　处：《西南林业大学学报（自然科学）》2017年第6期129-134,共6页Journal of Southwest Forestry University：Natural Sciences

基　　金：国家自然科学基金项目(31200265、31560106)资助;西南林业大学博士科研启动经费(111712)资助;云南省高校优势特色重点学科(生物学)建设项目资助;云南省林学一流学科建设经费资助;云南省高校森林有害生物科技创新团队项目资助;云南省森林灾害预警与控制重点实验室开放基金项目(ZK150009)资助

摘　　要：为开发环境友好型的植物源抗病毒剂,以烟草花叶病毒(TMV)为供试病毒,采用半叶枯斑法,研究了11种植物不同溶剂提取物的抗TMV活性。结果表明:当浓度为1 mg/m L时,臭灵丹石油醚提取物、红花月见草乙酸乙酯提取物、臭灵丹水层提取物对TMV增殖有显著抑制(P<0.05)活性抑制率分别为68.47%、80.19%、94.57%;臭灵丹石油醚提取物、钻叶紫菀乙酸乙酯提取物、臭灵丹水提取物对TMV有较强的钝化活性,抑制率分别为80.00%、76.38%、83.33%。TAS-ELISA检测表明,臭灵丹、风轮草、珊瑚樱、钻叶紫菀、益母草、拔毒散等植物提取物对普通烟K326内病毒增殖具有显著抑制作用(P<0.05),并且明显好于阳性对照药剂宁南霉素。In order to develop environmentally friendly ant/viral agents from plants, the anti tobacco mosaic vi- rus （TMV） activities of extracts from 11 plants were studied by half-leaf method. The results showed that petroleum ether extracts from Laggera pterodonta, the ethyl acetate extracts from Oenothera rosea and the water extracts from L. pterodonta showed potent antiviral proliferation activities with inhibition rates of 68.47%, 80. 19% and 94. 57% respectively at the concentration of 1 mg/mL. The petroleum ether extracts from L. pterodonta, ethyl acetate extracts from Aster subulatus and water extracts from L. pterodonta showed prominent inactivation activities against TMV with inhibition rate 80. 00%, 76. 38% and 83.33%, respectively. According to TAS-ELISA experiment, extracts from L. pterodonta , Clinopodium polycephalum , Solanum pseudo-capsicum, A. subulatus , Leonurus ArtemJzia , Sida szeehuensis showed significant anti-TMV proliferation activities in Nieotiana tabaeum CV. K326. The antivirus components from these plants were worth further investigation.

关 键 词：烟草花叶病毒 抗TMV活性 半叶枯斑法 叶圆片法 检测 

分 类 号：S718.85[农业科学—林学]