黑木耳部分种质资源SSR分子身份证的构建  

Establishment of a SSR Molecular ID System for Some Auricularia heimuer Germplasm Resources

作　　者：徐安然 付永平[1] 王延锋[2] 张志武 纪策 王菲[1] 叶健强 李玉[1,2] 宋冰 

机构地区：[1]吉林农业大学食药用菌教育部工程研究中心,长春130118 [2]黑龙江省农业科学院牡丹江分院,牡丹江157041 [3]华盛顿州立大学作物和土壤科学系,美国华盛顿州普尔曼99164

出　　处：《农业生物技术学报》2017年第12期1930-1939,共10页Journal of Agricultural Biotechnology

基　　金：国家重点基础研究发展规划(973)项目(No.2014CB138305);农业部公益性行业科研专项(No.201503137);吉林省秸秆综合利用技术创新平台(吉高平合字[2014]B-1);吉林农业大学校内启动基金(No.2015007);吉林省科技发展项目(No.20140101148JC);高等学校学科创新引智计划资助项目(No.D17014)

摘　　要：黑木耳(Auricularia heimuer)是我国各地广泛栽培的重要食用型菌类,在形态发育和遗传育种方面均备受关注,但黑木耳存在同物异名现象,这给育种工作带来了诸多不便。分子身份证是作物品种数字化的DNA指纹,在食用菌种质资源的鉴定和保护方面具有重要作用。本研究利用SSR标记对来源于全国不同区域的72份黑木耳栽培种和野生种菌株进行了遗传多样性分析及分子身份证的构建,从65对SSR引物中筛选出8对核心引物对收集来的种质资源进行了分析,利用非加权组平均法(unweighted pairgroup method with arithmetic means,UPGMA)对扩增结果进行了聚类分析,结果表明,供试菌株遗传相似性系数在0.37~1.00之间,在相似性0.62处可将供试菌株分为3大类群,Au1~Au11属于黑山系列,亲缘关系较近,可能存在同物异名的现象;Au44和Au90、Au40和Au73、Au22和Au60属于同物异名现象。根据不同代表菌株对SSR引物产生的不同扩增谱型,构建出了这72份不同的黑木耳菌株的分子身份证。本研究结果为食用菌种质资源鉴定和保护提供了依据。Auricularia heimuer is an important and widely cultivated edible fungus in China, and the morphological development and genetic breeding were concerned by researchers. It has brought a lot of inconvenience in breeding, because of introduction and serious synonymy phenomenon. A molecular ID system based on SSR markers for identifying and differentiating of germplasm resource, and it is important to identify and protect the germplasm resources of edible fungi. In this study, SSR markers were employed to clarify the genetic diversity and to establish the SSR ID of 72 accessions of A. heimuer collected from different areas in China, which contain cultivated and wild species. Eight SSR primer pairs were selectedfrom a pool of 65 primer pairs, the cluster results were analysed by the method of UPGMA（unweighted pairgroup method with arithmetic means）. The results showed that the genetic similarity coefficient were 0.37-1.00, at the 0.56, the tested strains could be divided into 3 groups. The following groups of strains are the synonyms. Au1 to Au11 is series of Hei Shan, Au44 and Au90, Au40 and Au73, Au22 and Au60. They have a close relationship. Based on the results of the UPGMA cluster analysis, amplification patterns SSR molecular ID system, the molecular identities for the resource protection and new varieties of A. heimuer can be construct. The results provide a basis for identification and protection of edible fungi germplasm resources.

关 键 词：黑木耳 分子身份证 SSR分子标记 

分 类 号：S325[农业科学—作物遗传育种]