盐和干旱胁迫下红麻HcWD40-1基因的克隆及表达特征  

Cloning and Expression Characteristics of HcWD40-1 Gene Under Salt and Drought Stress in Kenaf(Hibiscus cannabinus)

作　　者：李辉 李德芳[2] 陈安国[2] 唐慧娟[2] 李建军[2] 黄思齐[2] 

机构地区：[1]湖南文理学院生命与环境科学学院,常德415000 [2]中国农业科学院麻类研究所,长沙410205

出　　处：《农业生物技术学报》2017年第12期1970-1978,共9页Journal of Agricultural Biotechnology

基　　金：国家麻类产业技术体系红麻育种项目(No.CARS-19-E07);中国农业科学院科技创新工程一年生麻类育种项目(No.ASTIP-IBFC03)

摘　　要：WD-重复蛋白(WD-repeat protein)含有WD-40基序,又称为WD40重复蛋白,在植物对非生物逆境胁迫响应过程中具有重要的调控作用。红麻(Hibiscus cannabinus)是重要的工业纺织原料,是盐碱地土壤改良的先锋作物。本研究以红麻转录组中与WD40基因高度相似的Unigene为参考,设计引物,进行反转录PCR扩增,经Sanger测序获得基因Hc WD40-1(Gen Bank登录号:KX711617)的c DNA序列,生物信息学分析表明,该基因开放阅读框为1 356 bp,编码451个氨基酸,具有7个典型的WD40结构域。q RT-PCR分析表明,Hc WD40-1基因受盐和干旱胁迫诱导表达,同时受脱落酸(abscisic acid,ABA)和茉莉酸甲酯(methyl jasmonate,Me JA)的诱导。研究结果表明,Hc WD40-1基因是ABA和Me JA信号转导途径、盐和干旱胁迫应答途径的关键枢纽基因。该研究结果为红麻耐盐、抗旱的脱落酸信号调控网络和茉莉酸信号调控网络的研究提供基础资料。WD-repeat protein, containing WD-40 motif, also known as WD40 repeat protein, which plays an essential regulating role during the abiotic stress response of plants. In this study, it was found that a kenaf（Hibiscus cannabinus） transcriptome unigene which was highly similar to the WD40 gene, and then designed the primers to carry out reverse transcription PCR amplification and obtained HcWD40-1（Gen Bank No.：KX711617） c DNA sequence by sanger sequencing. Bioinformatics analysis showed that the gene open reading frame was 1 356 bp, encoding 451 amino acids, and containing 7 typical WD40 domains. qRT-PCR analysis showed that HcWD40-1 gene was induced by salt and drought stress, and was induced by abscisic acid（ABA） and methyl jasmonate（MeJA）. The results suggested that the HcWD40-1 gene was a key hub gene for ABA and MeJA signaling pathways, salt and drought stress responses. The results of the study provide basic data for the study of salt-tolerant, drought-resistant on ABA signal regulation network and MeJA signal regulation network of kenaf.

关 键 词：红麻 耐盐 抗旱 WD40重复蛋白 

分 类 号：S563.5[农业科学—作物学]