三个绵羊品种BMPR-IB基因CDS区1113位点的多态性与产羔数的相关性分析  

The Correlation Analysis Between Polymorphism and Fecundity of 1113 Locus in BMPR-IB Gene CDS Area of Three Sheep(Ovis aries) Varieties

作　　者：杜丽 张利平[1] 张筱艳[1] 朱韶华 马晓明 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《农业生物技术学报》2017年第12期1989-1997,共9页Journal of Agricultural Biotechnology

基　　金：甘肃农业大学科研项目(No.GSAU-ZL-2015-027);国家自然科学基金(No.31260547)

摘　　要：绵羊(Ovis aries)的多胎性状由主效基因控制,骨形态发生蛋白受体IB基因(bonemorphogenetic protein type IB,BMPR-IB)已被证实为多产母羊的主效基因之一。为寻找绵羊繁殖力的标记和育种提供理论依据,本研究采用聚合酶链反应-单链构象多态性分析法(single strand conformation polymorphism analysis of polymerase chain reaction products,PCR-SSCP)对3种绵羊(小尾寒羊48只;蒙古羊188只,其中包括100只单胎母羊,88只双胎母羊;甘肃高山细毛羊112只,其中包括70只单胎母羊,42只双胎母羊)BMPR-IB基因编码区1113位点进行单核苷酸多态性(single nucleotide polymorphism,SNP)检测,并分析3种绵羊BMPR-IB基因的多态性及其与产羔数之间的关联性,以期为研究绵羊高繁殖力的机理提供资料。结果表明,在蒙古羊单、双胎母羊和甘肃高山细毛羊单、双胎母羊中均发现3种不同基因型,分别为野生型AA、纯合突变型BB和杂合突变型AB,优势基因型为AB;小尾寒羊出现两种基因型,分别为纯合突变型BB和杂合突变型AB,优势基因型为BB;经多态信息含量(polymorphism information content,PIC)分析可知,小尾寒羊处于低度多态(PIC<0.25),甘肃高山细毛羊和蒙古羊均处于中度多态(0.25<PIC<0.5);BMPR-IB基因编码区1113位点突变在不同繁殖力绵羊品种间存在显著差异(P<0.05),该位点可能与绵羊产羔数有关,可作为繁殖性状的标记位点,本研究结果可进一步为生产实践提供理论依据。The fecundity of sheep（Ovis aries） is controlled by the dominant gene, and the bonemorphogenetic protein type IB gene（BMPR-IB） has been identified as one of the dominant genes in multiparous ewes. To provide a theoretical basis for breeding for sheep fecundity and markers, the polymerase chain reaction single strand conformation polymorphism（PCR） was used to analysis the single nucleotide polymorphism（SNP）detection at 1113 loci in the bonemorphogenetic protein type IB（BMPR-IB） gene coding region of 3 breeds of sheep, including 188 Mongolian sheep, 112 Gansu Alpine Merino sheep and 48 Small Tailed Han sheep, thenwe also analyze the correlation among the polymorphism of 3 breeds of sheep BMPR-IB gene and litter size, in order to provide the material for studying the mechanism of prolificacy in sheep. The result showed that 3 different genotypes were found in Mongolian sheep and Gansu Alpine Merino sheep, including wild type called AA, homozygous mutant called BB and heterozygous mutant called AB, AB was the dominant genotype. 2 different genotypes were found in Small Tailed Han sheep, BB and AB, BB was the dominant genotype. With the analysis of polymorphism information content, it was shown that Small Tail Han sheep belonged to low polymorphism（PIC〈0.25）, the other two breeds of sheep belonged to moderate polymorphism（0.25〈PIC〈0.5）. There was a significant difference in the 1113 locus of BMPR-IB gene coding region among these 3 different breeds of sheep, this site may be related to the litter size of sheep so it can be used as a marker loci for reproductive traits of sheep and further theoretical basis for the production practice.

关 键 词：骨形态发生蛋白受体IB基因(BMPR-IB) 小尾寒羊 蒙古羊 甘肃高山细毛羊 多态性 

分 类 号：S813.3[农业科学—畜牧学]