丁香假单胞菌Z2-6半连续发酵生产冠菌素的研究  

Study on the Production of Coronatine by Pseudomonas syringae Z2-6 Using Semicontinuous Fermentation

作　　者：于莎 姜峰 安冬捷 谭伟明 李召虎 段留生 

机构地区：[1]中国农业大学农学院/植物生理生化重点实验室/教育部植物生长调节剂工程研究中心,北京100193 [2]中国农业大学园艺学院,北京100193

出　　处：《农业生物技术学报》2017年第12期2066-2071,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(No.2011AA10A206)

摘　　要：冠菌素(coronatine,COR)是一种环境友好的新型植物生长调节剂,能有效调控植物生长,增强植物抗逆性。目前冠菌素主要通过微生物发酵的方式获得,而发酵效率低是限制冠菌素应用的最大问题。为提高冠菌素产量,本研究建立了丁香假单胞菌(Pseudomonas syringae)Z2-6的半连续发酵方式,并对移取起始时间、移取间隔时间以及移取体积3个方面进行了优化。结果表明,第10天开始移取较第8、9天开始移取可以延长冠菌素产出周期,使冠菌素合成始终维持在较高的水平。间隔1 d移取较间隔2 d移取冠菌素生产效率更高。当移取量不大于30%时,不会对菌体量产生影响,并且当移取量为30%时冠菌素产量达到最高。与传统发酵方法相比,在发酵第10天起每隔1 d移出30%发酵液并补充等体积新鲜培养基,可使冠菌素产量由11.2 mg/(L·d)提高到22.1 mg/(L·d),生产效率提高了98%。本研究利用半连续发酵方式有效提高了冠菌素发酵生产效率,节约了生产成本,为冠菌素的工业化生产和农业推广应用提供有力支持。Coronatine（COR） is a kind of novel plant growth regulator, which is environmentally friendly and can be used to effectively control plant growth and enhance plant resistance. At present, coronatine is mainly produced by microbial fermentation, however low fermentation yield is the biggest problem that limits the application of coronatine. To improve the yield of coronatine, the method of semicontinuous fermentation using Pseudomonas syringae Z2-6 was established, and related fermentation conditions were optimized.Optimization includes the initial removal time, and removal volume. The results showed that the beginning of removal at the 10 d post inoculate（dpi） could extend the production cycle of coronatine, which extended for more than 4 d than the beginning at 8 or 9 dpi, and kept the synthesis of coronatine maintaining at a high level. Coronatine production efficiency with time interval of 1 d was higher than 2 d. When the amount of removal was not more than 30%, the growth of bacteria was not be affected. The highest coronatine yield wasobtained at the amount of removal of 30%. In conclusion, when 30% of the fermentation broth was removed from 10 dpi every day and equal volume fresh medium was supplemented, the production of coronatine reached the highest level. The yield of coronatine was increased from 11.2 mg/（L· d） to 22.1 mg/（L· d）, and the production efficiency was increased by 98%. This method of semicontinuous fermentation could be used to improve the production efficiency of coronatine, reduce the production cost, and provide support for the further popularization of coronatine.

关 键 词：丁香假单胞菌 冠菌素 半连续发酵 

分 类 号：S182[农业科学—农业基础科学]