AQP5和CC16蛋白在大鼠失血性休克复苏后肺损伤中的表达及意义  

The expression and significance of protein AQP5 and CC16 in lung injury after hemorrhagic shock resuscitation in rats

作　　者：宋轶[1] 路晓光[1] 陈龙翊 范治伟[1] 康新[1] 白黎智[1] 王禹 刘杰 

机构地区：[1]大连大学附属中山医院急诊医学科,辽宁省大连116001 [2]临淄区人民医院,山东省淄博255400 [3]遵义医学院研究生院,贵州省遵义563003

出　　处：《中华急诊医学杂志》2017年第12期1397-1401,共5页Chinese Journal of Emergency Medicine

基　　金：国家自然科学基金（81673801,81473512,81173397,30971626）

摘　　要：目的观察大鼠肺脏水通道蛋白5（aquaporin5，AQP5）和克拉拉细胞蛋白16（Clara cell protein 16, CC16）表达变化，探讨失血性休克复苏后急性肺损伤发生的机制。方法健康清洁级雄性Sprague。Dawley大鼠，32只，随机（随机数字法）分为对照组和失血性休克复苏组两组（It=16），再按休克复苏后12h、24h分为两个亚组（n=8）。按Wiggers改良法制作失血性休克模型，将回收血液加等体积林格液回输进行复苏。取血测定血浆内毒素含量，酶联免疫法检测肺组织AQP5及血浆CC16蛋白含量。取左肺测湿／干质量比，苏木精伊红染色法观察肺组织病理改变，免疫组织化学法测定肺组织CC16蛋白和AQP5表达。结果①血浆内毒素含量：休克复苏组显著高于对照组（P〈0．01），且随时间递增。②血浆CC16蛋白含量：休克复苏组明显高于对照组（P〈0．05），且随时间递增。③肺组织AQP5含量：休克复苏组较对照组明显降低（P〈0．05），且随时间递减。④肺组织含水量（左肺湿／干比）检测：休克复苏组明显高于对照组（P〈0．05）。⑤肺组织苏木精伊红染色观察：对照组肺泡结构完整，肺泡囊清晰，肺泡间隔正常。休克复苏组肺泡间隔增宽，肺泡腔内有明显的出血渗出，中性粒细胞浸润。⑥肺组织CC16免疫组织化学评分：对照组明显高于休克复苏组（P〈0．05）。⑦肺组织AQP5免疫组织化学评分：对照组明显高于休克复苏组（P〈0．05）。结论AQP5及CC16蛋白参与失血性休克复苏后急性肺损伤发生过程，AQP5及CC16蛋白表达降低与时间呈正相关。Objective To observe the expression of protein AQP5 and CC16 in long after hemorrhagic shock resuscitation in rats in order to explore the mechanism of acute lung injury. Methods Thirty-two healthy and clean male SD rats were randomly （ random number） divided into two groups ： control group and hemorrhagic shock resuscitation group （n = 16 in each）. Besides, each group was further divided into two subgroups according to the experiment done at 12 h and 24 h after hemorrhagic shock resuscitation （n = 8 ）. The hemorrhagic shock model was made by using Wiggers＇ modified method. Resuscitation was done by transfusing the autologous blood and the equal volume of Ringer＇ s solution. Blood samples were obtained from abdominal aorta at each given interval to measure the level of plasma endotoxin, and assay the CC16 and AQP5 by using ELISA. After the rats were sacrificed, the left lung tissue was taken to measure lung water content and the dry/wet ratio, and to examine the levels of CC16 and AQP5 in lung tissue by immunohistochemical method. Results ①The level of plasma endotoxin in the experimental group was significantly higher than that in the control group （P 〈 0. 01 ） . ②The content of plasma CC16 in theexperimental group was higher than that in the control group （ P 〈 0. 05 ）. ③Compared with the control group, the content of pulmonary homogenate AQP5 in the experimental group was significantly lower （P 〈 0. 05） . ④The lung water content （the dry/wet ratio） of the experimental group was obviously higher than that of the control group （P 〈 0. 05）. ⑤Hislogogical observation with HE staining showed in the control group, the alveolar structure was complete, the alveolar sacs were clear, and the alveolar septum was intact; but in the experimental group, the alveolar septum was widened, and there were obvious hemorrhage and neutrophil infiltration in the alveolar space. ⑥ The level of lung tissue CC16 in control group was significantly higher compared with experimenta

关 键 词：失血性休克 全身炎症反应 急性肺损伤 水通道蛋白5 克拉拉细胞蛋白16 克 拉拉细胞 

分 类 号：R459.7[医药卫生—急诊医学]