乙肝病毒大蛋白检测在乙型肝炎诊断中的临床效果观察  

Clinical observation on effect of detection of hepatitis B virus large protein in diagnosis of hepatitis B

作　　者：张彬 王奎山 宋华之 

机构地区：[1]衡水市中心血站血液保障部,河北衡水053000

出　　处：《国际检验医学杂志》2017年第23期3257-3258,3261,共3页International Journal of Laboratory Medicine

基　　金：河北省衡水市科技支撑计划项目(2016014023Z)

摘　　要：目的比较常规检测方法和乙肝病毒大蛋白检测两种不同方法的临床效果,旨在提高乙肝的正确检出率。方法根据随机性原则选取2016年9月至2017年2月该院住院治疗的乙型肝炎患者80例作为研究对象,随机分为对照组40例,观察组40例。观察组患者采取乙肝病毒大蛋白检测方法进行检查,对照组患者采取常规检查方法进行检查,比较采取不同检查方法的患者的检出率。结果采用实时荧光定量PCR(Real-time PCR)进行检测的患者乙肝病毒检测阳性率为86.25%(69/80),较对照组采取酶联免疫吸附测定法(ELISA)检测的患者乙肝病毒检测阳性率72.50%(58/80)高,比较差异具有统计学意义(P<0.05);对照组HBV-DNA阳性率为77.50%(31/40),较观察组HBV-DNA阳性率90.00%(36/40)低,比较差异具有统计学意义(P<0.05)。结论常规检测方法和乙肝病毒大蛋白检测两种不同的检验手段都可检测出乙肝病毒,乙肝病毒大蛋白检测的检出率更高,为了尽量降低误诊率临床推荐使用检查乙肝病毒大蛋白。Objective To compare the clinical effect of the conventional detection and detection of hepatitis B virus large protein, aiming at improving the detection rate of hepatitis B. Methods According to the principle of random selection,80 cases of hepatitis B patients,from September 2016 to February 2017 in our hospital, were selected and were randomly divided into two groups,40 cases in the control group, 40 cases in the observation group. The observation group was adopted the detection of hepatitis B virus large protein while the control group were treated by conventional detection method. The detection rate of two groups was com-pared. Results The positive rate of hepatitis B virus infection by Real-timePCR for detection in observation group was 86. 25% （69/80） , which was higher than 72. 50% （58/80） in control group by ELISA,and the difference was statistically significant （P〈C 0. 05）. The positive rate of HBV-DNA in control group was 77. 50% （31/40） which was lower than 90. 00% （36/40） in observa-tion group,and the difference was statistically significant （P〈0 . 05）. Conclusion The conventional detection method and detection of hepatitis B virus large protein both can detect the hepatitis B virus,and detection rate of detection of hepatitis B virus large protein was higher. Detection of hepatitis B virus large protein is recommended for the detection of hepatitis B virus in order to reduce the misdiagnosis rate.

关 键 词：乙型肝炎 常规检测 乙肝病毒大蛋白 效果观察 

分 类 号：R446.6[医药卫生—诊断学] R512.62[医药卫生—临床医学]