活体外黄芪根腐病菌细胞壁降解酶产生能力比较  

Comparison of Production Capacity of Cell Wall Degrading Enzyme by Astragalus Root Rot Pathogens in Vitro

作　　者：岳换弟 秦雪梅[1] 王梦亮[2] 雷振宏 高芬[2] 

机构地区：[1]山西大学中医药现代研究中心,太原030006 [2]山西大学应用化学研究所,太原030006 [3]山西振东道地药材开发有限公司,山西长治047100

出　　处：《湖北农业科学》2017年第22期4328-4333,共6页Hubei Agricultural Sciences

基　　金：山西省中药现代化关键技术研究振东专项(2014ZD0501-2)

摘　　要：利用细胞壁降解酶(CWDEs)是镰刀菌(Fusarium sp.)侵染寄主的主要手段之一,不同种类的镰刀菌在致病过程中起主要作用的降解酶种类有所不同。以山西省黄芪(Astragalus L.)根腐病的优势病原菌锐顶镰刀菌[F.acuminatum(Ellis et Everhart)Wr.]、腐皮镰刀菌[F.solani(Mart)Sacc.]和尖孢镰刀菌(F.oxysporum Schlecht)为研究对象,对其活体外诱导培养产生的主要细胞壁降解酶(多聚半乳糖醛酸酶、聚甲基半乳糖醛酸酶、多聚半乳糖醛酸反式消除酶、果胶甲基反式消除酶、内切1,4-β-D葡聚糖酶、β-葡萄糖苷酶)及其变化规律进行了比较。结果表明,3种根腐病菌均能产生多聚半乳糖醛酸酶、聚甲基半乳糖醛酸酶、多聚半乳糖醛酸反式消除酶、果胶甲基反式消除酶、内切1,4-β-D葡聚糖酶和β-葡萄糖苷酶6种CWDEs,但不同的致病菌产生各种酶的活性大小和变化趋势具有明显差异。该结果为深入研究CWDEs在根腐病菌侵染黄芪过程中的致病作用奠定了基础。Producing cell wall degrading enzymes（CWDEs） is one of the main means of the Fusarium sp.infecting its host plants.Different kinds of Fasarium may have different CWDEs which are more active than other CWDEs in the infection process.In this paper,research was made into the main CWDEs produced by the dominant pathogens of Astragalus L.root rot in Shanxi province such as F.acuminatum（Ellis et Everhart） Wr.,F.solani（Mart） Sacc.and F.oxysporum Schlecht and the rules of their changes.A series of CWDEs including polygalacturonase（PG）,polymethylgalacturonase（PMG）,polygalacturonic acid transeliminase（PGTE）,pectin methyltranseliminase（PMTE）,carboxymethyl cellulose（Cx）,β-glucosidase（βG）,were detected by the induction and culture in vitro.The results show that the 3 kinds of root rot pathogens all produced PG,PMG,PGTE,PMTE,Cx and βG,but significant differences existed in the activities and dynamic changes of each of the various enzymes produced by these pathogens.The above results laid the foundation for further study on the pathogenicity of CWDEs in the infection of root rot pathogens.

关 键 词：黄芪根腐病菌 细胞壁降解酶 产生能力 致病作用 

分 类 号：S435.672.22[农业科学—农业昆虫与害虫防治]