激光扫描共聚焦显微镜使用中荧光共定位的一种计算方法  

A calculation method of fluorescent colocalization for confocal laser scanning microscope

作　　者：胡西学[1] 郭宏博[1] 甘雅玲[1] 

机构地区：[1]国家纳米科学中心,北京100190

出　　处：《电子显微学报》2017年第6期577-581,共5页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金资助项目(No.31200717)

摘　　要：激光扫描共聚焦显微镜在形态学、分子细胞生物学、神经科学和药理学等研究领域得到广泛地应用,荧光共定位计算是该仪器的一种重要应用功能。共定位计算的结果为肿瘤治疗过程中药物与肿瘤的作用机理研究提供了依据之一,推动了肿瘤精准定位治疗的发展。随着科技的进步和科研的深入,人们对共定位的分析结果要求越来越高。本文提出的激光共聚焦显微镜荧光共定位计算方法,通过3D构建和反卷积,获得了更加真实的荧光共定位计算结果。Confocal laser scanning microscopy(CLSM) has a wide range of applications in many fields including the morphology,molecular cell biology,neuroscience and pharmacology. The precise treatment for tumors has recently become the trend of biomedical development and the colocalization of drugs and cells is one of the most important means during the research. The overlap efficiency of drugs and cells gives a basis for researching the mechanism of tumors and drugs during the process of tumor treatment,promoting the development of precise treatment for tumors. The result of colocalization is required to be more precise with the progress of science and technology. In this paper,a calculation method of fluorescent colocalization for confocal laser scanning microscope is proposed with 3 D construction of samples and deconvolution algorithm and more realistic fluorescent colocalization calculation results can be obtained.

关 键 词：激光共聚焦扫描显微镜 荧光共定位计算方法 共定位系数 3D构建 反卷积 

分 类 号：Q6-33[生物学—生物物理学]