肿瘤坏死因子α基因启动子多态性与泛发性脓疱性银屑病相关性研究  

Association of tumor necrosis factor-a gene promoter polymorphisms with generalized pustular psoriasis

作　　者：龙福泉 刘业强 钱伊弘 蒙秉新[3] 赵丽诗 王千秋[4] 

机构地区：[1]上海市皮肤病医院性病科,200043 [2]上海市皮肤病医院病理科,200043 [3]海南省人民医院皮肤科 [4]中国医学科学院北京协和医学院皮肤病研究所性病临床防治室

出　　处：《中华皮肤科杂志》2017年第12期879-882,共4页Chinese Journal of Dermatology

基　　金：上海市自然科学基金(14ZR143700);海南省自然科学基金(813219)

摘　　要：目的 探讨肿瘤坏死因子α（TNF？α）基因启动子区域多态性与泛发性脓疱性银屑病的相关性。方法 检测对象为91例汉族泛发性脓疱性银屑病患者及102例汉族健康体检者，应用PCR及直接测序法分析TNF？α基因启动子区域？238、？308、？857位点多态性。结果 泛发性脓疱性银屑病患者与健康对照组TNF？α？238位点G/A等位基因频率差异有统计学意义（P = 0.003；OR = 4.819，95% CI：1.581 ~ 14.694），基因型GG与GA/AA在两组间的分布差异也有统计学意义（P = 0.006；OR = 4.455，95% CI：1.410 ~ 14.077）；TNF？α？308位点G/A等位基因频率以及基因型GG与GA/AA的分布在两组间差异无统计学意义（P值分别为0.794、0.786）；TNF？α？857位点C/T等位基因频率以及基因型CC与CT/TT的分布在两组间差异无统计学意义（P值分别为0.474、0.453）。结论 TNF？α？238G 〉 A多态性可能与泛发性脓疱性银屑病发病相关。Objective To investigate the association of tumor necrosis factor-α （TNF-α） gene promoter polymorphisms with generalized pustular psoriasis. Methods Totally, 91 patients of Han nationality with generalized pustular psoriasis （generalized pustular psoriasis group） and 102 health checkup examinees （healthy control group） were enrolled into this study. PCR and direct sequencing were performed to analyze the -238, -308 and -857 polymorphic sites of the TNF-α promoter. Results The frequency of the A allele at TNF-α-238 site was significantly higher in the generalized pustular psoriasis group than in the healthy control group （P = 0.003, OR = 4.819, 95% CI： 1.581 - 14.694）, so was the frequency of GA/AA genotype （P = 0.006, OR = 4.455, 95% CI： 1.410 - 14.077）. However, no significant differences were observed in the frequencies of G/A alleles （P = 0.794） and GG/GA/AA genotypes （P = 0.786） at TNF-α-308 site, or in the frequencies of C/T alleles （P = 0.474） and CC/CT/TT genotypes （P = 0.453）at TNF-α-857 site, between the generalized pustular psoriasis group and healthy control group. Conclusion TNF-α-238G 〉 A polymorphisms may be associated with the occur-rence of generalized pustular psoriasis.

关 键 词：银屑病 肿瘤坏死因子Α 多态性 单核苷酸 转录启动子 泛发性脓疱性银屑病 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]