慢性自发性荨麻疹患者外周血Treg细胞比例和白细胞介素17A、27水平及意义  

Proportion of regulatory T cells and expression of interleukin-17A and -27 in the peripheral blood of patients with chronic spontaneous urticaria and their significance

作　　者：王亚婷[1] 石春蕊[1] 武奕 师蓓 熊潇[1] 周兰霞[1] 

机构地区：[1]兰州大学第一临床医学院兰州大学第一医院皮肤性病科,730000

出　　处：《中华皮肤科杂志》2017年第12期916-919,共4页Chinese Journal of Dermatology

基　　金：甘肃省卫生行业科研计划管理项目（GWGL2014-60）

摘　　要：目的 探讨外周血中CD4＋CD25＋CD127low调节性T（Treg）细胞、白细胞介素17A（IL？17A）和IL？27在慢性自发性荨麻疹发病机制中的作用。方法 收集37例慢性自发性荨麻疹患者和40例健康对照外周血标本。采用流式细胞仪检测外周血中CD4＋CD25＋CD127low Treg细胞比例，酶联免疫吸附试验（ELISA）检测血清中IL？17A和IL？27水平。结果 慢性自发性荨麻疹患者外周血中CD4＋CD25＋CD127low Treg细胞比例（5.99% ± 2.72%）明显低于健康对照组（9.07% ± 3.44 %，t = 4.325，P 〈 0.01），IL？27水平［（20.54 ± 7.65）ng/L］亦低于健康对照组［（26.63 ± 9.72） ng/L，t = 3.039，P = 0.003］，IL？17A表达水平与健康对照组差异无统计学意义（P = 0.529）。慢性自发性荨麻疹患者IL？17A表达水平与CD4＋CD25＋CD127low Treg细胞比例呈负相关（r = -0.359，P = 0.029），IL？17A、IL？27、CD4＋CD25＋CD127 low Treg细胞比例与UAS评分均无显著相关性（r值分别为-0.076、-0.083、-0.053，均P 〉 0.05）。结论 慢性自发性荨麻疹患者外周血中可能存在Th17/Treg细胞失衡，且IL？27可能参与慢性自发性荨麻疹的发病过程。Objective To explore the role of peripheral blood CD4＋CD25＋CD127low Treg cells, interleukin-17A （IL-17A） and IL-27 in the pathogenesis of chronic spontaneous urticaria （CSU）. Methods Peripheral blood samples were obtained from 37 patients with CSU （CSU group）and 40 healthy controls （control group）. Flow cytometry was performed to determine the percentage of CD4＋CD25＋CD127low Treg cells in the peripheral blood, and enzyme-linked immunosorbent assay （ELISA） to detect the serum levels of IL-17A and IL-27. Results The percentage of CD4＋CD25＋CD127low Treg cells in the peripheral blood （5.99% ± 2.72% vs. 9.07% ± 3.44 %, t = 4.325, P 〈 0.01） and the serum level of IL-27 （20.54 ± 7.65 ng/L vs. 26.63 ± 9.72 ng/L, t = 3.039, P = 0.003） were both significantly lower in the CSU group than in the control group. However, there was no significant difference in the serum level of IL-17A between the 2 groups （P = 0.529）. Among the patients with CSU, the level of IL-17A was negatively correlated with the percentage of CD4＋CD25＋CD127low Treg cells （r = -0.359, P = 0.029）, while the urticaria activity score （UAS） was uncorrelated with the levels of IL-17A and IL-27 as well as the percentage of CD4＋CD25＋CD127low Treg cells （r = -0.076, -0.083, -0.053 respectively, all P 〉 0.05）. Conclusion There may be Th17/Treg imbalance in the peripheral blood of patients with CSU, and IL-27 may be involved in the occurrence of CSU.

关 键 词：荨麻疹 白细胞介素 17 T淋巴细胞 调节性 白细胞介素 27 

分 类 号：R758.24[医药卫生—皮肤病学与性病学]