新罗丹明酰胺基硫脲荧光探针在水溶液中对Hg^(2+)的识别  

Rhodamine thiosemicarbazide probes for detecting Hg^(2+) in aqueous solution

作　　者：时蕾[1] 鹿泽华 龚毅君[1] 毕晶晶[1] 麻娜娜[1] 徐晶晶 张贵生[1] 

机构地区：[1]河南师范大学化学化工学院河南省有机功能分子创制与药物创新重点实验室,新乡453007

出　　处：《分析试验室》2017年第12期1365-1370,共6页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(21605039;21603062);河南省高等学校重点科学研究计划(15A150013;17A350006);河南省软科学研究计划(15B610005);河南师范大学博士启动课题(qd15108);河南师范大学青年科学基金资助项目(2016QK10)资助

摘　　要：设计并合成了一类罗丹明酰胺基硫脲Hg^(2+)荧光探针,通过~1HNMR谱和^(13)C谱进行结构表征。采用紫外-可见光谱和荧光光谱研究了探针RFS对Hg^(2+)的光谱响应。结果表明,探针罗丹明B酰胺基邻氟苯基硫脲(RFS)在水溶液中(HEPES缓冲溶液pH7.2)对Hg^(2+)表现出高选择性,并具有快速、灵敏的显色和Off-On荧光增强效应。探针与Hg^(2+)作用后,溶液颜色由无色变为红色,在1~10μmol/L范围内,探针的荧光强度与Hg^(2+)浓度呈线性关系。比较了因取代基的电子效应不同对探针灵敏度的影响,通过激光共聚焦成像实现了探针RFS在Hela细胞内对Hg^(2+)的识别检测,表明探针RFS可用于检测细胞内的汞离子。Three of rhodamine-base fluorescent probes, which were confirmed by 1HNMR and 13 CNMR detection were designed and synthesized. The interactions between Hg2＋ and probes were investigated via UV absorption spectroscopy and fluorescence spectrum. The results showed that the probe RFS has good selectivity, rapid, sensitive and off-on fluorescence responses to Hg2＋ in water. After the addition of Hg2＋ , the probes solution changed from colorless to red and showed a good linear relationship with Hg2＋ in the range of 1 - 10 μmol/L. Additionally, the probes have the detection limit of 0. 76 × 10 -7, 0. 6× 10-7 and 1.7 × 10-7 mol/L, respectively, suggesting their high sensitivities for Hg2＋ detection and showed that the different substituent＇ s electonic effect had influence on the sensitivity of the three probes. The probe RFS was successfully used to detect Hg2＋ in Hela cells in aqueous solution by laser confocal scanning microscope.

关 键 词：罗丹明酰氨基硫脲 Hg2+荧光探针 水溶液 HELA细胞 

分 类 号：O621.2[理学—有机化学]