高压电烧伤大鼠血小板流变行为变化及乌司他丁的干预效果  

Changes of platelet rheological behavior and the interventional effects of ulinastatin in rats with high- voltage electrical burns

作　　者：张庆富[1] 李勇[1] 冯建科[1] 徐彦粉[1] 屠丽红[1] 

机构地区：[1]河北医科大学第一医院烧伤整形外科河北省烧伤救治工程技术研究中心,石家庄050031

出　　处：《中华烧伤杂志》2017年第12期744-749,共6页Chinese Journal of Burns

基　　金：河北省自然科学基金重点项目（H2016206575）;河北省应用基础研究计划重点基础研究项目（14967724D）;河北省省级重大医学科研课题（zd2013080）

摘　　要：目的探讨高压电烧伤对大鼠血小板聚集数、β血小板球蛋白（β-TG）及血小板因子4（PF-4）的影响以及乌司他丁的干预效果。方法将240只SD大鼠按随机数字表法分为假伤组、单纯电伤组、生理盐水组和乌司他丁组，每组60只。以左前肢近心端外侧为电流入口，右后肢近心端外侧为电流出口，单纯电伤组、生理盐水组和乌司他丁组大鼠用调压器及实验变压器造成入口和出口处各1 cm×1 cm的高压电烧伤创面；假伤组大鼠接相同装置但不通电致假伤。伤后2 min，生理盐水组大鼠按2 mL/kg腹腔注射生理盐水，乌司他丁组大鼠按2×104 U/kg腹腔注射10 g/L乌司他丁。伤前15 min及伤后5 min、1 h、2 h、4 h、8 h，每组各取10只大鼠，经心脏采血5～7 mL，取0.05 mL制成活血涂片，观察血小板聚集数；其余血样分离血清，用ELISA法检测血清β-TG和PF-4含量。对数据行析因设计方差分析、SNK检验、Kruskal-Wallis H检验、Wilcoxon秩和检验，并进行Bonferroni校正。结果（1）伤前15 min，假伤组、单纯电伤组、生理盐水组、乌司他丁组大鼠血小板聚集数相近，分别为（5.9±1.2）、（5.8±1.2）、（5.9±1.3）、（5.9±1.1）个（P值均大于0.05）。伤后5 min、1 h、2 h、4 h、8 h，单纯电伤组大鼠血小板聚集数分别为（57.2±16.3）、（59.1±16.9）、（60.8±20.6）、（83.6±24.9）、（83.4±30.3）个，较假伤组的（6.0±1.3）、（6.0±1.4）、（5.9±1.4）、（5.7±1.1）、（5.8±1.3）个明显增多（P值均小于0.001）；乌司他丁组大鼠血小板聚集数分别为（29.6±7.4）、（31.9±10.1）、（35.0±14.2）、（43.0±13.6）、（35.2±11.1）个，明显少于生理盐水组的（58.3±16.1）、（63.9±18.0）、（60.8±17.7）、（74.2±23.0）、（82.3±21.9）个（P值均小于0.001）。假伤组大鼠组内各时相点血小板聚集数比较，差异均无统计学意义（P值均大于0.05）；其ObjectiveTo explore the influence of high-voltage electrical burns on the number of platelet aggregation, β-thromboglobulin （β-TG） and platelet factor 4 （PF-4） and the interventional effects of ulinastatin in rats with high-voltage electrical burns.MethodsA total of 240 Sprague-Dawley rats were divided into sham injury （SI） group, simple electrical burn （SEB） group, normal saline （NS） group, and ulinastatin （UTI） group according to the random number table, with 60 rats in each group. The electrical current was applied to the outside proximal part of left forelimb of rats and exited from the outside proximal part of right hind limb of rats. Rats in groups SEB, NS, and UTI were inflicted with high-voltage electrical burn wounds of 1 cm×1 cm at current entrances and exits, with the voltage regulator and experimental transformer. Rats in group SI were sham injured through connecting the same equipments without electricity. At 2 min post injury, rats in group NS were intraperitoneally injected with 2 mL/kg NS, and rats in group UTI were intraperitoneally injected with 2×104 U/kg UTI of 10 g/L. At 15 min before injury and 5 min, 1 h, 2 h, 4 h, 8 h post injury, 10 rats in each group were selected to collect 5-7 mL blood of heart respectively. Blood of 0.05 mL were collected to make fresh blood smear for observing the number of platelet aggregation, and serum were separated from the remaining blood to determine content of β-TG and PF-4 with enzyme-linked immunosorbent assay. Data were processed with analysis of factorial design of variance, student-Newman-Keuls test, Kruskal-Wallis H test, Wilcoxon rank sum test, and Bonferroni correction.Results（1） At 15 min before injury, the numbers of platelet aggregation of rats were close among groups SI, SEB, NS and UTI （5.9±1.2, 5.8±1.2, 5.9±1.3, 5.9±1.1, respectively, with P values above 0.05）. At 5 min, 1 h, 2 h, 4 h, 8 h post injury, the numbers of platelet aggregation of rats in group SEB were 57.2±16.3, 59.1±16.9, 60.8±20.6, 83.6±

关 键 词：烧伤 电 微循环 血小板聚集 血小板因子4 β血小板球蛋白 乌司他丁 

分 类 号：R647[医药卫生—外科学]